The aim of this study was to analyze kinetic and metabolic features of two hybridomas for monoclonal antibodies (MAbs) production for blood typing. Two lines of hybridomas, ED7 and ED9, were evaluated to produce anti-A and anti-AB, respectively. Experiments with ED7 and ED9 lines were conducted in a 500mL spinner flask and 2L bioreactor, using two different basal culture media, both with 10 % v/v addition of fetal bovine serum. The results revealed the necessity of additional supplementation of the culture media to meet the nutritional demands of the cells. Through nutritional balance and cultivation in better controlled conditions in the bioreactor, it was possible to raise the maximum specific growth rate µ max in 8.2 % and the productivity of anti-A in 148 % with the line ED7. For the ED9 line the µ max decreased 9.5% but the productivity of anti-AB increased 33.3%.