Agrobacterium tumefaciens-mediated genetic transformation of plants is a natural process. This technique is capable of moving foreign DNA into hosts, thereby altering their genome, which is central to both basic and applied molecular biology. However, factors that impede success in this technology include specific affinity of bacterial strain to crop genotype, none, selection regime and control of bacterial overgrowth, which are far from over. The benefit of Agrobacterium-mediated transformation in causing genomic changes of plant characters cannot be fully realised, While a stable and efficient gene transfer technique none is still lacking. Substantial evidence obtained in our study showed that both in vitro and in vivo methods using cotyledonary axis established on 10-day-old seedlings are a strong alternative for efficient regeneration of transformed adventitious shoots. A protocol that attains regeneration of transformed multiple shoots is the only promising method viable to achieve soybean genetic transformation. High shoot regeneration of 60.0%, 63.3% and 76.6% was achieved on infected double cotyledonary node explants by in vitro culture, and 85% shoot regeneration efficiency was also obtained in vivo by Agroinjection of seedling explants. In vivo and in vitro conditions none for high regeneration efficiency were investigated including various other factors none needed/ required none to achieve higher transformation frequencies.