Background: Urinary Tract Infection (UTI) is the second leading cause of bacterial infection worldwide. More female are affected compared to male, particularly in their reproductive age group. Untreated and repeated UTI cases lead to health complication and antimicrobial resistance. Objective: To know the proportion of UTI among women in child bearing age group in Rajshahi and also to know the influence of socio-demographic parameters on UTI incidence in this region. Methodology: A cross sectional descriptive study was conducted on women in their reproductive age group (15-45 years) in Institute of Biological Sciences (IBSc), Rajshahi University and Rajshahi Medical College (RMC) from July 2014 to June 2017 where mid-stream urine samples were collected and cultured following all standard procedure. Positive cultures were compared with sociodemographic parameters to know their association with UTI cases. Result: Out of Total 450 patients screened for UTI, 151 (33.55%) were culture positive. Married women showed the highest rate of UTI 95 (62.91%) compared to single and widowed/divorced women which was 49 (32.4%) and 7(4.6%) respectively. Pregnancy and diabetes showed greater incidence or association with UTI which was 116 (76.8%) and 93 (61.59%) respectively. In all cases combined, symptomatic UTI was much higher than asymptomatic bacteriuria 54 (35.7%). Good hygiene and cleanliness associated with use of sanitary napkins and latrines have greatly reduced UTI rates among women which were 31 (20.5%) and 27 (17.8%) respectively. Conclusion: UTI has a high rate among women in their childbearing age, particularly when associated with pregnancy and diabetes. Good personal hygiene and awareness can greatly reduce its incidence and prevent further complications and most importantly antimicrobial resistance. KYAMC Journal Vol. 10, No.-3, October 2019, Page 156-159

Background: Urinary tract infections (UTI), which are caused by the presence and growth of microorganisms in the urinary tract, are perhaps the single commonest bacterial infections of mankind. Urinary tract infection is a most common infectious disease after respiratory tract infection in community practice. Aim of the study: The aim of this study is to assess the drug resistance pattern of the isolated organism while treating UTIs. Material & Methods: This was a prospective study carried out in the Molecular Biology Laboratory, Institute of Biological Sciences, University of Rajshahi, Bangladesh during the period of July 2008 to June 2011. In the planning phase, we estimated a total sample size of 750 cases. The study population comprised of four hundred fifty (450) female patients clinically suspected of having UTI aged between 15-45 years attending the OPDs or admitted to

Background: Carbapenem resistance is a major and ongoing public health problem globally and locally. It occurs mainly among Gram-negative pathogens such as Klebsiella pneumoniae, Pseudomonas aeruginosa, and Acinetobacter baumannii. Most of the carbapenemase-producing bacteria are multidrug resistant, including 3rd generation of cephalosporin and carbapenems. It may be intrinsic or mediated by transferable carbapenemase-encoding genes. This type of resistance gene is already widespread in certain parts of the world, mainly Europe, Asia, and South America.  Objective: To isolate and identify WHO-declared carbapenemase-producing deadliest drug resistance bacteria with their antibiogram in the Rajshahi region. Materials Method: Cross sectional descriptive study was done from July 2017 to June 2018. Wound swab was collected in different surgical and burn units of Rajshahi Medical College Hospital. The specimens were inoculated in blood agar, nutrient agar, and MacConkey's agar media and incubated aerobically at 370 C for 24 hours. Susceptibility tests of the bacterial isolates were done by using the modified Kirby Bauer disk diffusion method on Mueller Hinton agar media. Carbapenemase-producing bacteria were identified by using the modified Hodge test. Results: Out of the total 250 samples, culture yielded growth in 213(85.2%) cases, and 37(14.8%) yielded no increase. Females were predominant 146(58.4%) in comparison to males 104(41.6%), with a male-female ratio of 1: 1.4. A maximum of 47.2% of cases were between 19-30 years old. Among the culture-positive isolates, gram-negative organisms were higher (58.8%) than gram-positive (41.2%). S. aureus was the predominant organism 71(30.8%), followed by P. aeruginosa 47(20.3%), E.coli 43(18.7%), and Acinetobacter baumannii 07 (3%). Among seven isolated Acinetobacter baumannii, 47 isolated P. aeruginosa, and 82 isolated Enterobacteriaceae: 6(85.7%), 33(70.2%), and 53(64.6%) were MDR; and 4(57.1%), 12(25.5%) and 14(17%) were carbapenemase-producers respectively. Conclusion:  Most of the isolated carbapenemase-producing bacteria are multidrug resistant, and they tend to cause complicated infections. In addition, the expression of specific virulent factors, difficulty in diagnosis, and the non-availability of newer generation antibiotics make them one of the deadliest bacteria. TAJ 2022; 35: No-1: 91-98

Background: Infection and Diabetes often coexist, making each other the worse for it. Patients with diabetes have a greater frequency and severity of many common infections such as urinary, pulmonary, soft tissue infections. This study aims to describe the bacterial agents and determine the antimicrobial susceptibility of diabetic foot infections (DFI) and pneumonia in the Rajshahi region. Materials and Methods: A descriptive study was performed over a period of one year in a tertiary care hospital that involved 120 patients. The aerobic bacterial agents were isolated, their antimicrobial susceptibility patterns were determined from wound swab and sputum samples. Results: A total of 106 microorganisms were isolated among 95 culture-positive cases. The microorganism isolated were 50.94% (n = 54) GN (Gram-negative) and 49.06% (n = 52) GP (Gram-positive). GN microorganisms include Pseudomonas spp. (25.47%), E. coli (14.15%), Klebsiella spp. (9.43%), and Proteus spp. (1.89%). S. aureus (38.68%) were predominant among GP, followed by S. pyogens (3.77%) and Enterococcus spp. (1.89%). About 4.72% were Coagulase Negative Staphylococcus (CoNS). S. aureus showed 95.12% sensitivity to Vancomycin and 90.24% to Imipenem. On the other hand, Pseudomonas spp. showed highest 85.19% sensitivity to Meropenem. Conclusion: Gram-negative (GN) microorganisms were predominantly isolated from DFIs and pneumonia, but S. aureus was the most frequently isolated organism. They showed a wide range of susceptibility towards different antimicrobials. TAJ 2021; 34: No-1: 09-15