Background: Pre selection of baby gender is still desire of many parents. A number of procedures have been used including X and Y sperm separation by various semen processing procedures for intrauterine insemination (IUI), sperm sorting by flowcytometry, fluorescent in situ hybridization and preimplantation genetic testing (PGT). Due to low cost, simplicity and easy availability, various X-and Y-bearing sperm separation procedures followed by IUI have been commonly used but with conflicting accuracy. The most common sperm separation procedures include; gradients filtration, swim up, swim down and albumin gradients filtration. The objective of this study was to investigate whether swim-up or density gradient semen processing followed by IUI results in significant deviation in the ratio of male and female babies. Methods: The study was carried out retrospectively at Thuriah Medical Center. The data of 990 IUI cycles from Jan 2016 to Dec 2017 were analyzed. The semen samples were processed by gradient filtration, swim up or simple wash depending on quality of the sample and desire of patient. Data on female and male age, total progressively motile sperm inseminated, pregnancy rate and delivery of male or female baby were recorded and analyzed. Results: Gradient filtration, swim up and simple wash were used in 69, 26 and 5 % samples, respectively. Male or female ages in different groups were not significantly different (P<0.05). Biochemical pregnancy rates were highest in the swim up group and lowest in the simple wash group. Conclusion: Based on the results of this study, the gender ratio did not change significantly from the natural ratio of about 50/50; therefore, sperm separation by these procedures for gender selection is not accurate.