Ammonia-treated bagasse with 80%(w/w) moisture content was subjected to mixed-culture solid-substrate fermentation (SSF) with Trichoderma reesei LM-UC4 and Aspergillus phoenicis QM 329, in flask or pot fermenters, for cellulase production. Significantly higher activities of all the enzymes of the cellulase complex were achieved in 4 days of mixed-culture SSF than in single-culture (T. reesei) SSF. The highest filter-paper-cellulase and β-glucosidase activities seen in mixed-culture SSF were 18.7 and 38.6 IU/g dry wt, respectively, representing approx. 3- and 6-fold increases over the activities attained in single-culture SSF. The mixed-culture SSF process also converted about 46% of the cellulose and hemicellulose to reducing sugars and enriched the product with 13% fungal protein. The biomass productivity, 0.29 gl(-1).h, and enzyme productivity, 28.0 IU I(-1).h, were about twice as high in the mixed-culture than in the single-culture.
In order to guarantee the required level of quality for our Bone &Tissue Banking, we evaluated a new CE marked container (CELLFLEX MacoPharma), for packing, transport, processing and storage of bones for therapeutic use. The use of CE marked containers is mandatory for organ and tissue containers (Medical Device Directive 93/42). We carried out a three-phase study: (1)Evaluation, (2) Implementation, (3)Audit The product was evaluated for the following criteria:Dash mechanical resistance, Dash air tightness, Dash fragility, Dash capacity. No damage was observed after the storage period, even after immersion in liquid nitrogen. No breakages were observed after provoked impact tests (pots dropped onto the floor). The pot capacity evaluation showed that the inner pot volume (approximately 500 ml) permits adequate storage of tendons and the majority of bone allografts. In conclusion, this evaluation has shown that the CELLFLEX kit is suitable for long duration preservation of bone allografts even at very low temperature conditions (vapour phase nitrogen). Its format and structure permit preservation of most bone allografts.
Se mezcló pulpa kraft blanqueada de Pinus douglasiana con médula blanqueada de bagazo de caña Saccharum officinarum y se formaron hojas estándar de acuerdo con las normas TAPPI. Para evaluar estadísticamente los efectos de la adición de médula sobre el largo de ruptura y el índice de rasgado en hojas estándar se aplicó un diseño experimental factorial (3x5) (factor A = grado de refinación de la pulpa: 12 °SR, 30 °SR y 60 °SR; factor B = cantidad de médula: 0%, 10%, 20%, 30% y 40%). Los resultados indican que la médula de bagazo de caña ayuda a incrementar el largo de ruptura, sobre todo en las pulpas con 12 °SR y 30 °SR. Se encontraron diferencias estadísticas para ambas variables entre la pulpa con 12 °SR y las refinadas a 30 °SR y 60 °SR; mientras que, estadísticamente, los resultados de las pulpas refinadas a 30 °SR y 60 °SR fueron similares.
En el presente trabajo se dan a conocer los resultados de un estudio de las características macroscópicas y microscópicas de la madera de Persea americana var. guatemalensis, Mill (Hass) procedente de plantaciones comerciales (huertas), motivado principalmente por la situación que prevalece en torno al cultivo y producción de aguacate en México y por ende, a la disponibilidad de esta madera, que es actualmente desaprovechada. Este trabajo forma parte de un estudio integral sobre las propiedades tecnológicas de la madera de esta especie. En función de las relaciones morfológicas de sus fibras, se hace un análisis tendiente a visualizar las características y propiedades de pulpas celulósicas resultantes, ante un eventual aprovechamiento para la producción de celulosa para papel. Se incluye información general sobre la distribución geográfica, taxonomía y reportes sobre otros estudios y aprovechamiento de este recurso forestal.
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