Lipids, including the diterpenes cafestol and kahweol, are key compounds that contribute to the quality of coffee beverages. We determined total lipid content and cafestol and kahweol concentrations in green beans and genotyped 107 Coffea arabica accessions, including wild genotypes from the historical FAO collection from Ethiopia. A genome-wide association study was performed to identify genomic regions associated with lipid, cafestol and kahweol contents and cafestol/kahweol ratio. Using the diploid Coffea canephora genome as a reference, we identified 6,696 SNPs. Population structure analyses suggested the presence of two to three groups (K = 2 and K = 3) corresponding to the east and west sides of the Great Rift Valley and an additional group formed by wild accessions collected in western forests. We identified 5 SNPs associated with lipid content, 4 with cafestol, 3 with kahweol and 9 with cafestol/kahweol ratio. Most of these SNPs are located inside or near candidate genes related to metabolic pathways of these chemical compounds in coffee beans. In addition, three trait-associated SNPs showed evidence of directional selection among cultivated and wild coffee accessions. Our results also confirm a great allelic richness in wild accessions from Ethiopia, especially in accessions originating from forests in the west side of the Great Rift Valley.

a b s t r a c tCyclic ADP-ribose (cADPR) metabolism in mammals is catalyzed by NAD glycohydrolases (NADases) that, besides forming ADP-ribose, form and hydrolyze the N 1 -glycosidic linkage of cADPR. Thus far, no cADPR phosphohydrolase was known. We tested rat ADP-ribose/CDP-alcohol pyrophosphatase (ADPRibase-Mn) and found that cADPR is an ADPRibase-Mn ligand and substrate. ADPRibase-Mn activity on cADPR was 65-fold less efficient than on ADP-ribose, the best substrate. This is similar to the ADP-ribose/cADPR formation ratio by NADases. The product of cADPR phosphohydrolysis by ADPRibase-Mn was N 1 -(5-phosphoribosyl)-AMP, suggesting a novel route for cADPR turnover.

The low aqueous solubility of most hydrophobic medications limits their oral absorption. An approach to solve this problem is to make a drug−polymer association. Herein, we investigated the association between rafoxanide (RAF), a surface-active, poorly water-soluble drug, with a commercial hydrophilic polymer povidone. We found that the association is a function of medium composition and could only take place in polar media, such as water. The association is favored by the hydrogen-bond formation between the amide group in RAF and the carbonyl group in povidone. In addition, the association is also favored by the self-association of RAF through π−π interaction between the benzene rings in adjacent RAF molecules. Twodimensional nuclear magnetic resonance has been applied to investigate the interactions and has confirmed our hypotheses. Geometry optimization confirmed that RAF exists primarily in the antiparallel configuration in the RAF aggregates. This study provides critical information for designing suitable drug−vehicle complexes and engineering the interactions between them to maximize the oral absorption. Our results shed light on drug design and delivery, drug molecule structure−functionality relationship, as well as efficacy enhancement toward interaction engineering.

Knowledge of the spatial variability of soil properties and of forage yield is needed for informed use of soil inputs such as variable rate technology (VRT) for lime and fertilizers. The objective of this research was to map and evaluate the spatial variability of soil properties, yield, lime and fertilizer needs and economic return of an alfalfa pasture. The study was conducted in a 5.3 ha irrigated alfalfa pasture in Sao Carlos, SP, Brazil that was directly grazed and intensively managed in a 270-paddock rotational system. Alfalfa shoot dry matter yield was evaluated before grazing. Soil samples were collected at 0-0.2 m depth, and each sample represented a group of 2 or 3 paddocks. Apparent soil electrical conductivity (ECa) was measured with a contact sensor. The cost of producing 1 ha of alfalfa was estimated from the amount of lime and fertilizer needed and was then used to estimate the total cost of production for the dairy system. The alfalfa dry matter yield was used to simulate the pasture stocking rate, milk yield, gross revenue and net profit. The spatial variability of soil properties and site-specific liming and fertilizer needs were modeled using semi-variograms with VESPER software, the soil fertility information and economic return were modeled with SPRING software. The results showed that geostatistics and GIS were effective tools for revealing soil and pasture spatial variability and supporting management strategies. Soil nutrients were used to classify the soil spatial distribution map and design site-specific lime and fertilizer application maps. Spatial variation in forage and spatial estimates of stocking and milk yield are adequate pasture management tools. Spatial analyses of needs, forage availability and economic return are management tools for avoiding economic problems, as well as potential environmental problems, caused by unbalanced nutrient supplies and over-or under-grazing.

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