Lung adenocarcinoma (LUAD) accounts for the most common histological subtype of lung cancer which remains the leading cause of cancer death worldwide. The discovery of more sensitive and specific novel target biomarkers for predicting the development and progression of LUAD is imperative. Flotillin-1 (Flot-1) has been reported to have important roles in the progression of several tumor types but not been reported in the progression of LUAD. Here, we demonstrated that the expression of flotillin-1 was upregulated in 5 LUAD cells. Moreover, multiple approaches were used to explore the tumorigenicity of flotillin-1 in LUAD cell lines. The expression levels of flotillin-1 were analyzed by immunoblotting after overexpression and siRNA-based knockdown. Cell proliferation, scratch wound healing, transwell migration and matrigel invasion and xenograft tumor growth assays were used to determine the role of flotillin-1 in LUAD progression. Downregulation of flotillin-1 reversed, whereas upregulation of flotillin-1 enhanced, the malignant phenotype of LUAD cells in vitro. Consistently, cells with flotillin-1 knockdown formed smaller tumors in nude mice than cells transfected with the empty vector. Furthermore, the control group demonstrated significantly more tumorigenic effects compared to the flotillin-1-silenced group in the xenograft model of LUAD. In all, there draws a conclusion that flotillin-1 is a tumorigenic protein that plays an important role in promoting the proliferation and tumorigenicity of LUAD, suggesting that flotillin-1 may represent a novel the therapeutic target to LUAD.

In order to test and verify the accuracy and reasonable of the mathematical model of the high-pressure air blowing the main ballast tanks, the paper design a submarine self-propelled model with the system of high-pressure air blowing the ballast tanks.Through the experiment without propulsion , the paper obtains drainage performance and key performance parameters of motion.Through the comparing of CFD, the result shows the ability to describe correctly the process of high-pressure air blowing ballast tanks.

The Three Gorges ship lock is an important part of the Three Gorges project, its completion and using have greatly improved transport conditions of the Yangtze River. With the development of the Yangtze River shelter-forest economy and water transport, the great pressure has been brought to the Three Gorges ship lock, the "bottlenecks phenomenon" of the Three Gorges ship lock is prominent increasingly. In order to improve the navigation capacity of the Three Gorges existing ship lock, and ensure navigation pass through ship lock to be convenient, safe, smooth and orderly, based on analyzing the navigation capacity of the ship lock and its main influencing factors, this paper establish ant colony optimization model for the ship lock arrangement, meantime to design and implement the corresponding algorithm. Computational results show that the application of the ant algorithm to the ship lock arrangement optimization is effective, and it offers a new way to solve this kind of optimization problem, moreover, it also offers technical support for improving the navigation capacity of the Three Gorges existing ship lock.

A set of structural composition and system composition of self-propelled submarine model which has already been used in experiments as well as the details of its long-wave controller and automation systems was introduced in this study. The feasibility of this technique applied to the model has been proved by real test in lake and the results derived from that will have some guiding significance to research on maneuverability of submarine which thus indicates that keep developing the self-propelled submarine model technique will for sure has a bright future.

The applications of the 3D design are researched in the design of submarine self-propelled model in the paper. By analyzing the experimental instances, the methods and ideas of 3D design are given in the design of the submarine self-propelled model. There is of great significance in improving the effect of mechanical design, enhancing design efficiency and shorten the cycle of designing and processing.