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Objective: The aim of this study was to obtain the time range of non-industrial fresh watermelon juice (FWJ), which is widely used in the catering industry under different storage conditions, with safe-drinkable quality, and the drinking time range of fresh juice with good nutritional quality and sensory quality. Method: The quality of non-industrial FWJ was audited by assessing the shelf life of non-industrial FWJ through microbial safety, nutritional, and sensory quality investigating during 24 h of storage at 4, 25, and 37°C. Results: According to the microbial safety quality, the safe drinking time of FWJ was within 12, 4, and 4 h when stored at 4, 25, and 37°C, respectively. Based on the nutritional and sensory quality, FWJ was drinking with good quality within 2 h, and with just acceptable quality for no more than 4 h when stored at 4 or 25°C. Electronic nose and gas chromatography-mass spectrometry (GC-MS) could effectively distinguish and identify the changes in volatile components in FWJ under different storage conditions. Conclusion: It is a feasible method to predict the shelf life of non-industrial FWJ by this method, and hence to guarantee non-industrial FWJ being drinking with safety and health, and it might be used in many other fresh juice shelf life predictions. Popular scientific summary • The shelf life of fresh watermelon juice were predicted. • The safe drinking time of FWJ was within 12, 4, and 4 h when stored at 4, 25 and 37°C. • The drinking time of FWJ with good quality was within 2 h when stored at 4°C or 25°C. • The FWJ still had acceptable quality when stored at 4°C or 25°C for no more than 4 h. • E-nose and GC-MS could distinguish and identify the changes in volatile in FWJ.

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