Stemona japonica is a species of perennial, flowering and herbaceous vines in the genus Stemona and the family Stemonaceae. In this study, we presented and annotated the complete chloroplast genome of S. japonica, which the chloroplast genome length was 154,224 bp, containing a large single-copy region (LSC) of 82,118 bp, a small single-copy region (SSC) of 17,938 bp and two same inverted repeat regions (IR) of 27,084 bp. The cp genome of S. japonica comprised 134 genes that included 88 proteincoding genes (PCGs), 38 transfer RNA (tRNA) genes and 8 ribosomal RNA (rRNA) genes. The overall nucleotide of cp genome composition is: A of 47,338 bp (30.7%), T of 48,206 bp (31.3%), C of 29,528 bp (19.1%), and G of 29,101 bp (18.9%), with a total A þ T content of 62.0% and G þ C content of 38.0%. Phylogenetic Neighbor-Joining (NJ) analysis based on 11 plants species chloroplast genomes with Stemona japonica, the result shown that Stemona japonica is closely related to Stemona mairei in evolutionary relationship.

IntroductionOsteosarcoma (OS) is one of the most common malignant bone tumors, with an incidence of 4-5 per million among children and teenagers. Panax notoginseng saponins (PNS) a derivatives from Panax notoginseng, are potent drugs that have many biological activities including antitumor effects. However, there have been no reports focused on the effect of PNS on OS development.Material and methodsMTT and flow cytometry was used to detect the proliferation and apoptosis of OS cells treated by PNS. The expression of miR-760 was identified by qPCR. Luciferase assay was performed to verify the target of miR-760. Western blot was used to detect the expression of target proteins. In vivo analysis was employed to confirm the antitumor effect of PNS.ResultsWe tested the PNS effect on a large numbers of microRNAs (miRs) in OS cells, we found that PNS significantly reduced miR-760. Also, luciferase assay has shown SMAD4 to be the target gene of miR-760 in OS cells. Rescue experiments were carried out to verify the relation between SMAD4 and miR-760. we found that overexpression of miR-760 can reverse the effect of PNS. PNS proven to exerts its effect through miR-760. Moreover, SMAD4 can reverse the effect of miR-760, indicating that miR-760 targets SMAD4 in OS cells.ConclusionsThis study extends our understanding of the effect of PNS in OS cell. we revealed a novel signaling pathway involved in the PNS mode of action, miR-760/SMAD4, this new pathway might be feasible as a target for the treatment of OS.

The peel of Citrus reticulata can be useful as a kind of traditional Chinese medicine in China. The circular complete chloroplast genome of Citrus reticulata was 160,101 bp in length with the typical quadripartite structure of angiosperms, including 87,721 bp large single-copy region (LSC), 18,394 bp small single-copy region (SSC) of and a pair of 26,993 bp inverted repeat (IR) regions. The overall nucleotide composition of chloroplast genome sequence is 30.5% A, 31.0% T, C (19.6%), G (18.9%), and the total G þ C content of 38.5%. A total of 138 genes were annotated in chloroplast genome of C. reticulata. The phylogenetic tree result showed that C. reticulata was evolutionarily close to Citrus sinensis in the family Rutaceae the genus Citrus by the maximum-likelihood (ML) method.