Epithelial ovarian cancer (OC) is the most deadly cancer of the female reproductive system. To date, there is no effective screening method for early detection of OC and current diagnostic armamentarium may include sonographic grading of the tumor and analyzing serum levels of tumor markers, Cancer Antigen 125 (CA-125) and Human epididymis protein 4 (HE4). Microorganisms (bacterial, archaeal, and fungal cells) residing in mucosal tissues including the gastrointestinal and urogenital tracts can be altered by different disease states, and these shifts in microbial dynamics may help to diagnose disease states. We hypothesized that the peritoneal microbial environment was altered in patients with OC and that inclusion of selected peritoneal microbial features with current clinical features into prediction analyses will improve detection accuracy of patients with OC. Blood and peritoneal fluid were collected from consented patients that had sonography confirmed adnexal masses and were being seen at SIU School of Medicine Simmons Cancer Institute. Blood was processed and serum HE4 and CA-125 were measured. Peritoneal fluid was collected at the time of surgery and processed for Next Generation Sequencing (NGS) using 16S V4 exon bacterial primers and bioinformatics analyses. We found that patients with OC had a unique peritoneal microbial profile compared to patients with a benign mass. Using ensemble modeling and machine learning pathways, we identified 18 microbial features that were highly specific to OC pathology. Prediction analyses confirmed that inclusion of microbial features with serum tumor marker levels and control features (patient age and BMI) improved diagnostic accuracy compared to currently used models. We conclude that OC pathogenesis alters the peritoneal microbial environment and that these unique microbial

In network analysis, the core structure of modeling interest is usually hidden in a larger network in which most structures are not informative. The noise and bias introduced by the non-informative component in networks can obscure the salient structure and limit many network modeling procedures' effectiveness. This paper introduces a novel core-periphery model for the non-informative periphery structure of networks without imposing a specific form for the informative core structure. We propose spectral algorithms for core identification as a data preprocessing step for general downstream network analysis tasks based on the model. The algorithm enjoys a strong theoretical guarantee of accuracy and is scalable for large networks. We evaluate the proposed method by extensive simulation studies demonstrating various advantages over many traditional core-periphery methods. The method is applied to extract the informative core structure from a citation network and give more informative results in the downstream hierarchical community detection.

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In a complex network, the core component with interesting structures is usually hidden within noninformative connections. The noises and bias introduced by the noninformative component can obscure the salient structure and limit many network modeling procedures’ effectiveness. This paper introduces a novel core–periphery model for the noninformative periphery structure of networks without imposing a specific form of the core. We propose spectral algorithms for core identification for general downstream network analysis tasks under the model. The algorithms enjoy strong performance guarantees and are scalable for large networks. We evaluate the methods by extensive simulation studies demonstrating advantages over multiple traditional core–periphery methods. The methods are also used to extract the core structure from a citation network, which results in a more interpretable hierarchical community detection.