Hemodialysis is a kidney function replacement therapy that aims to remove metabolic waste or certain toxins from human blood circulation. Patients with chronic renal failure who undergo hemodialysis therapy will experience various problems such as psychological disorders, including Self-Concept Disorders, Anxiety, Depression, and Grieving. This aims of study to determine the relationship between anxiety, stress and fatigue in hemodialysis patients. The design of this study was a cross sectional study. The sample studies consisted of 37 patients undergoing hemodialysis. the inclusion criteria were aged over 17 years, signed informed consent. Exclusion criteria were patients with neurological disorders and speech difficulties. The results of the study were more female respondents (56.8%) than male respondents. Higher education respondents were 64.9% more dominant than those with low education. The average age was 52.16 years, with the lowest age being 24 years and the oldest being 78 years. The average length of hemodialysis was 32.95 months with the shortest being 1 month and the longest being 168 months. The average score of anxiety was 10.11 with the lowest score of 0 and the highest was 20. The average score of stress was 13.54 with the lowest score of 0 and the highest score of 27. Average fatigue score of 20.7 with the lowest score of 9 the highest was 34. Anxiety (p = 0,001) and Stress (p=0,024) correlated with fatigue. The relationship between stress and fatigue shows a moderate relationship and a positive pattern, which means that the higher the stress level, the higher the patient's fatigue. The test results showed a significant relationship between stress and fatigue in patients. The relationship between anxiety and fatigue shows a moderate relationship with a positive pattern, meaning that the more anxious patients undergoing hemodialysis are, the more fatigue they are. Therefore, it can be concluded anxiety are determinant factor of fatigue in hemodyalisis patients.