Cellulose and chitosan are natural polymers that have been used as biocomposite. The aim of this research is to obtain biofilms from chitosan and oxidized cellulose crosslinks. This research is divided into three steps, i.e., isolation of cellulose from oil palm trunk and oxidation of cellulose using NaIO4 (0.2; 0.4; 0.6; 0.8; 1.0 mg/mL) to obtain dialdehyde cellulose (DAC), crosslink of oxidized cellulose with chitosan (DD = 72.85%) to obtain biofilm of chitosan/DAC (CDAC), and characterization of biofilms. The crosslinked reaction was confirmed by FT-IR analysis that showed the spectrum of Schiff base C=N group at 1651 cm–1. Tensile strength increased gradually when the NaIO4 concentration used was 0.2–0.6 mg/mL, but after those concentrations, the tensile strength slightly decreased. The morphology analysis showed that CDAC had smoother morphology than DAC, which was shown rough and showed some particle indicated the presence of unreacted cellulose. CDAC biofilms that prepared with 1.0 mg/mL NaIO4 showed the greatest antibacterial activity.

Research on antimicrobial and toxicity tests of flavonoid total Dendrophthoe pentandra (L) Miq from false ashoke tree (Polyalthia longifolia) have been done. This study aims to determine the activity of toxicity of total flavonoid of Dendrophthoe pentandra (L) Miq as mistletoe of false ashoke tree (P. longifolia) based on Brine Shrimp Lethality Test (BSLT) method using Artemia salina Leach shrimp larvae obtained Lethal Concentration (LC 50) of 30.06 mg / L which means strong toxic activity. Antimicrobial test of flavonoid total has used the diffusion method in order to obtain the inhibit zone diameter on Streptococcus mutans at the total flavonoids concentration of 3%, 6% and 9% were 6; 9 and 17.25 mm, in Escherichia coli is 3.55; 4.25 and 9.15 mm and at Candida albicans is 8.30; 4 and 5.30 mm where S. mutans and E. coli are greater but C. albicans less affect but still have good inhibitory activity so effective to be developed as antimicrobial agents.

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