Fusarium graminearum is the major causal agent of Fusarium head blight (FHB) disease in cereal crops worldwide. Infection with this fungal phytopathogen can regularly cause severe yield and quality losses and mycotoxin contamination in grains. In previous other studies, one research group reported that pyrrolnitrin had an ability to suppress of mycelial growth of F. graminearum. Other groups revealed that phenazine-1-carboxamide, a derivative of phenazine-1-carboxylic acid, could also inhibit the growth of F. graminearum and showed great potentials in the bioprotection of crops from FHB disease. In our recent work with Pseudomonas chlororaphis strain G05, however, we found that although the phz operon (phenazine biosynthetic gene cluster) was knocked out, the phenazine-deficient mutant G05Δphz still exhibited effective inhibition of the mycelial growth of some fungal phytopathogens in pathogen inhibition assay, especially including F. graminearum, Colletotrichum gloeosporioides, Botrytis cinerea. With our further investigations, including deletion and complementation of the prn operon (pyrrolnitrin biosynthetic gene cluster), purification and identification of fungal compounds, we first verified that not phenazines but pyrrolnitrin biosynthesized in P. chlororaphis G05 plays an essential role in growth suppression of F. graminearum and the bioprotection of cereal crops against FHB disease.
Mosquito light traps for household use are popular because they are small, cheap, user friendly, and environment friendly. At present, there are many variations and specifications of mosquito traps intended for household use on the market. The light traps claim they are powerful, but research and evaluation are lacking. Key parameters such as capture rates in the laboratory and field of 5 popular mosquito traps were evaluated as intended for household use. This study found that in the laboratory experiments, the capture rate of the mosquito traps selected was between 34.7% and 65.0%. Field tests in greenhouses found that the 5 mosquito traps had high catch rates for Culex quinquefasciatus. The percentage of Cx. quinquefasciatus, Aedes albopictus, Anopheles sinensis, and other flying insects captured was 51.76%, 25.29%, 14.12%, and 8.82%, respectively. There was no significant difference in the capture rate of Ae. albopictus and An. sinensis by the 5 mosquito traps in the greenhouse, but a significant difference in the catch rate of Cx. quinquefasciatus. The analysis showed that the fan speed and design of the air guide of the traps are important factors that affect the mosquito catch rate and that the ultraviolet wavelength (395–400 nm) used by the traps did not impact mosquito catch rates. Therefore, the mosquito traps intended for household use can be improved by adjusting the fan speed and optimizing the air guide.
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