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Vegetation water consumption in the Sanjiangyuan Region is of direct significance to the utilization of local water resources. To measure the actual evapotranspiration of various typical vegetation with different vegetation types in the Sanjiangyuan Region, a Lysimeter was used between November 2019 and October 2020. Additionally, the Penman–Monteith equation was used to estimate the condensation water of different vegetation types. Based on the measured data, this paper analyzes the spatial distribution of annual water consumption and annual runoff of various vegetation types. Furthermore, the spatial and temporal distribution of monthly water consumption of vegetation types on different underlying surfaces are discussed. To establish the relationship between the precipitation and runoff of various vegetation types, an artificial rainfall test was conducted. This study’s results reveal several key findings: (1) Condensation water is widespread and can be observed throughout the year. The annual condensation water volume ranges between 28.47 and 56.88 mm, which is particularly significant for the growth of alpine desert steppe and alpine steppe vegetation. (2) The annual water consumption in the Sanjiangyuan Region was higher in the south than in the north. Shrub water consumption was found to be 58.1–73.3 mm higher than that of grasses. Water consumption primarily occurred during the growing season, spanning from May to October. (3) The total water consumption in the growing season of the alpine meadow was less affected by precipitation compared to the non-growing season (from November to the next April). (4) The runoff yield can be ignored in the non-growing season when calculating water balance. However, during the growing season, the calculation of runoff cannot be ignored due to its significant impact on vegetation water consumption.

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