Nasopharyngeal carcinoma (NPC) is a malignant epithelial tumor originating in the nasopharynx and has a high incidence in Southeast Asia and North Africa. To develop these comprehensive guidelines for the diagnosis and management of NPC, the Chinese Society of Clinical Oncology (CSCO) arranged a multi-disciplinary team comprising of experts from all sub-specialties of NPC to write, discuss, and revise the guidelines. Based on the findings of evidencebased medicine in China and abroad, domestic experts have iteratively developed these guidelines to provide proper management of NPC. Overall, the guidelines describe the screening, clinical and pathological diagnosis, staging and risk assessment, therapies, and follow-up of NPC, which aim to improve the management of NPC.
BackgroundLung adenocarcinoma is the most common pathological type of lung cancer. However, the mechanisms underlying its development are still poorly understood. B7-H3 was discovered as a new member of the B7 costimulatory family.MethodsWe detected the expression status of B7-H3 protein in lung adenocarcinoma tissues, and evaluated the relationship of B7-H3 expression and patients’ prognosis. Then, we silenced its expression in A549 cells by transient siRNA transfection to ascertain the function of B7-H3 in lung adenocarcinoma cells. Western blotting was used to detect the expression of epithelial–mesenchymal transition (EMT) related proteins.ResultsWe found that B7-H3 overexpressed in lung adenocarcinoma. It is correlated with lymph node metastasis, distant metastasis, and disease stage. The Cox regression analysis showed that B7-H3 might serve as an independent prognostic marker of lung adenocarcinoma. We also found that B7-H3 promoted proliferation, invasion and migration of A549 cells in vitro. B7-H3 also could promote EMT progression by regulating EMT-related molecules.ConclusionB7-H3 is a potential target for the treatment of lung adenocarcinoma.
The detection of Epstein-Barr virus (EBV) DNA load in nasopharyngeal (NP) brushing samples for diagnosis of nasopharyngeal carcinoma (NPC) has attracted great attention. Further improvements that eliminate the need for clinical settings will greatly extend its application. A total of 250 participants were recruited to obtain NP brushing samples. Brush sampling both with and without the guide of endoscopy was conducted in 38 NPC patients. EBV DNA load, EBV RNA transcript and EBV DNA C promoter methylation status were, respectively, evaluated. Typical latency II transcripts were observed in brushing samples from NPC patients but not controls. Unlike in tissues, multiple lytic gene transcripts were observed not only in NPC patients but also in controls. Apart from EBV RNA transcript, samples from NPC patients also showed higher levels of EBV DNA load and C promoter methylation degree than their controls. Qualitative analysis further showed that EBV DNA C promoter was methylated in all NPC patients but in only 18.4% of the control group. Combined analysis of EBV DNA methylated degree and EBV DNA load increased the sensitivity to 100% in the detection of NPC. Using qualitative methylated type as the criteria, up to 89.5% of samples collected via blind brushing showed consistent results with samples collected via endoscopy-guided brushing from NPC patients. Detection of the methylation status of EBV DNA C promoter in NP brushing samples shows great potential in diagnosing NPC and may provide an appealing alternative for the non-invasive detection and screening of NPC without the need for clinical settings. K E Y W O R D Sdiagnosis, Epstein-Barr virus DNA load, Epstein-Barr virus DNA methylation, nasopharyngeal brushing, nasopharyngeal carcinoma 1 | INTRODUC TI ON Nasopharyngeal carcinoma (NPC) is a highly invasive and metastatic cancer that is widely prevalent in southern China. Although the overall survival rate is approximately 90% in patients diagnosed with early clinical stage disease, unfortunately, most patients are diagnosed with advanced stage diseased at their first visit to the hospital, and the survival rate decreases to <50%. 1 Previous studies have S U PP O RTI N G I N FO R M ATI O NAdditional supporting information may be found online in the Supporting Information section.
No abstract
Abstract. The present study aimed to characterize the expression of Krüppel-like factor 8 (KLF8) in nasopahryngeal carcinoma (NPC) cell lines and determine its effect on tumor development and invasion following KLF8 gene knockdown by small hairpin RNA (shRNA). KLF8 expression in four NPC cell lines was examined by quantitative polymerase chain reaction (qPCR) and western blotting. KLF8 was knocked down in the SUNE1-5-8F/Sh-KLF8 cell line using shRNA, and the resulting stable cell line SUNE1-5-8F-sh-KLF8 was transplanted into nude mice in order to observe tumor formation and invasion. The results obtained from qPCR and western blotting revealed that, of the four NPC cell lines, KLF8 expression was lowest in the CNE-1 cells and highest in the SUNE1-5-8F cells. The tumor xenograft mouse models revealed that SUNE1-5-8F/Sh-KLF8 cells had a reduced ability for tumor formation and invasion compared with the control group. These results demonstrated for the first time that KLF8 modulates the formation and invasive ability of nasopharyngeal carcinoma.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
customersupport@researchsolutions.com
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.