Summary Despite decades of studies meant to analyse the bacterial response to carbon limitation, we still miss a high‐resolution overview of the situation. All gene expression changes observed in such conditions cannot solely be accounted for by the global regulator Crp either free or bound to its effector, cyclic AMP . Here, for the first time, we evaluated the response of both CDS (protein‐coding sequence) and nc RNA (non‐coding RNA ) genes to carbon limitation, revealed cellular functions of differentially expressed genes systematically, quantified the contribution of Crp‐ cAMP and other factors to regulation and deciphered regulation strategies at a genomewide scale. Approximately one‐third of the differentially expressed genes we identified responded to Crp‐ cAMP via its direct or indirect control, while the remaining genes were subject to growth rate‐dependent control or were controlled by other regulators, especially RpoS. Importantly, gene regulation mechanisms can be established by expression pattern studies. Here, we propose a comprehensive picture of how cells respond to carbon scarcity. The global regulation strategies thus exposed illustrate that the response of cell to carbon scarcity is not limited to maintaining sufficient carbon metabolism via cAMP signalling while the main response is to adjust metabolism to cope with a slow growth rate.

Small non-translated regulatory RNAs control plenty of bacterial vital activities. The small RNA GcvB has been extensively studied, indicating the multifaceted roles of GcvB beyond amino acid metabolism. However, few reported GcvB-dependent regulation in minimal medium. Here, by applying a high-resolution RNA-seq assay, we compared the transcriptomes of a wild-type Escherichia coli K-12 strain and its gcvB deletion derivative grown in minimal medium and identified putative targets responding to GcvB, including flu, a determinant gene of auto-aggregation. The following molecular studies and the enhanced auto-aggregation ability of the gcvB knockout strain further substantiated the induced expression of these genes. Intriguingly, the reduced expression of OxyR (the oxidative stress regulator) in the gcvB knockout strain was identified to account for the increased expression of flu. Additionally, GcvB was characterized to up-regulate the expression of OxyR at the translational level. Accordingly, compared to the wild type, the GcvB deletion strain was more sensitive to oxidative stress and lost some its ability to eliminate endogenous reactive oxygen species. Taken together, we reveal that GcvB regulates oxidative stress response by up-regulating OxyR expression. Our findings provide an insight into the diversity of GcvB regulation and add an additional layer to the regulation of OxyR.

How bacteria adjust gene expression to cope with variable environments remains open to question. Here, we investigated the way global gene expression changes in E. coli correlated with the metabolism of seven carbon substrates chosen to trigger a large panel of metabolic pathways. Coarse-grained analysis of gene co-expression identified a novel regulation pattern: we established that the gene expression trend following immediately the reduction of growth rate (GR) was correlated to its initial expression level. Subsequent fine-grained analysis of co-expression demonstrated that the Crp regulator, coupled with a change in GR, governed the response of most GR-dependent genes. By contrast, the Cra, Mlc and Fur regulators governed the expression of genes responding to non-glycolytic substrates, glycolytic substrates or phosphotransferase system transported sugars following an idiosyncratic way. This work allowed us to expand additional genes in the panel of gene complement regulated by each regulator and to elucidate the regulatory functions of each regulator comprehensively. Interestingly, the bulk of genes controlled by Cra and Mlc were, respectively, co-regulated by Crp-or GR-related effect and our quantitative analysis showed that each factor took turns to work as the primary one or contributed equally depending on the conditions.

Since the appearances of “extraordinary” gains and losses in the authoritative GAAP literature in 1917, the debate over how to report such items has continued for a century. Until the 1970s, the reporting of extraordinary items was widespread. During recent decades, however, the frequency of reported extraordinary items decreased sharply. Only 1.5 percent of companies reported them in 2014, followed in January 2015 by their elimination from U.S. GAAP. In this paper, we investigate factors affecting the decline in reporting of extraordinary items and reasons for their elimination from U.S. GAAP. Factors include evolving criteria for defining extraordinary items, variation in their financial statement placement, and their changing nature and size over the years. We examine several important FASB pronouncements and events that contributed to the elimination of extraordinary items, including APB Opinion No. 30 in 1973, the FASB's treatment of the losses from the World Trade Center attack in 2001, the FASB and IASB's convergence initiative, the FASB's simplification initiative, and widespread use of pro forma earnings in practice.

The construction of digital twin cities is a current research hotspot. Video data are one of the important aspects of digital twin cities, and their digital modeling is one of the important foundations of its construction. For this reason, the construction and digital analysis of video data space has become an urgent problem to be solved. After in-depth research, this study found that the existing video space construction methods have three shortcomings: first, the problem of high requirements for objective conditions or low accuracy; second, the lack of easy and efficient mapping algorithms from 2D video pixel coordinates to 3D; and third, the lack of efficient correlation mechanisms between video space and external geographic information, making it difficult to integrate video space with external information, and thus prevent a more effective analysis. In view of the above problems, this paper proposes a video stereo grid geometric space construction method based on GeoSOT-3D stereo grid coding and a camera imaging model to form a video stereo grid space model. Finally, targeted experiments of video stereo grid space geometry construction were conducted to analyze the experimental results before and after optimization and compare the variance size to verify the feasibility and effectiveness of the model.