No abstract

Simple, sensitive and accurate UV spectrophotometric method for simultaneous determination of Rosuvastatin and Telmisartan has been developed. The new method was developed with Methanol and phosphate buffer (pH 7.2) in proportion of 30:70 as solvent system. The several HPLC and UV spectroscopic methods were reported in literature for estimation of Rosuvastatin and Telmisartan. Therefore need to develop simple, more sensitive and economic method for simultaneous for estimation of Rosuvastatin and Telmisartan in bulk and in combined dosage form is described. Rosuvastatin showed at 276.30 nm and Telmisartan showed at 295.70nm. Linearity range was observed in the concentration range of 5-25µg/ml for Rosuvastatin calcium and 10-50µg/ml for Telmisartan. Percentage purity and recovery study were in the limit of 98-102% for both drugs. Limit of Detection for Rosuvastatin and Telmisartan were found to be 0.508µg/ml and 1.54µg/ml respectively. Limit of Quantitation for Rosuvastatin and Telmisartan were found to be 1.402 µg/ml and 4.25µg/ml respectively. The proposed method can be successfully used for the analysis of pure drug and marketed formulation. The method is found to be precise, simple, and accurate and can be applied for the routine estimation of Rosuvastatin and Telmisartan.

To develop an environment-safe aqueous solubility enhancement method of poorly water-soluble drugs is the need of the pharmaceutical field. Because when organic solvents were used for solubility enhancement, there are many disadvantages like carcinogenicity, environment pollutant, flammable, toxicity, and cost. The hydrotropic method has been used to enhance the aqueous solubility of poorly water-soluble drugs. Dolutegravir is slightly soluble in water. To enhance aqueous solubility various hydrotropes were used and optimized the concentration of each hydrotrope. From these hydrotropes, a mixture of 10% sodium tricitrate and 10% sodium benzoate was selected because Dolutegravir was completely soluble in a mixed hydrotropic solution. Method development and analytical validation were performed. The maximum wavelength of Dolutegravir in the hydrotropic mixture was found a 268nm, and the linearity curve in the range of 5-25µg/ml. A regression coefficient was found to be 0.999. Percent label claim, accuracy (% RSD) were found 99.58%, 0.13(80%), 0.11(100%), 0.13(120%), respectively. The LOD for Dolutegravir was determined to be 0.037μg/ mL, and LOQ was found to be 0.11μg/mL.