Background: This search was carried to investigate the phytochemical of Petroselinum sativum L.parsley and the antioxidant effect of parsley volatile oils leaves extract on oxidative stress [MDA (Malondialdehyde enzyme), LPO ( lipid peroxidation enzyme ) level and antioxidant glutathione enzyme (GSH)] in adult male albino rats.Objective: This research work aimed to study the antioxidant effect of volatile oils of parsley leaves extract against environment-induced free radicals oxidative stress in male albino rats. Methods: 30 male adult rats were used. Rats were divided into five Groups: G1 (Control group) received only 0.9% normal saline, G2 received P.sativum L volatile oils extracts 50 Mg / Kg B.W, G3-received P.sativum L volatile oils of extracts 100 mg/kg B.W, G4-received P.sativum L volatile oils of extracts 200 mg/kg B.W, G5-received P.sativum L volatile oils extracts 250 mg/kg B.W volatile oils of extracts. The extract was injected (i.p.) in some rats for 20 days and 30 days and 60 days. Then blood samples were collected for measuring MDA, LPO and GSH levels in experimental groups and compered with control. Results: Results indicated that MDA and LPO levels were significantly decreased at concentrations of 50, 100, 200 and 250 mg/kg B.W., while GSH levels significantly increased at the same concentrations compared to the control group. Conclusions: There was a dose-dependent marked decrease in the levels of MDA and LPO and a dose-dependent marked increase glutathione in male rats treated with parsley extract.