Trichoderma spp. is one of the biocontrol agents that have important benefits for agriculture in Indonesia. Exploration is carried out at PT Bumitama Gunajaya Agro Oil Palm Plantations in high yield blocks. This study aims to identify and determine the isolates of Trichoderma spp. and to find out its in vitro antagonism against Ganoderma spp. Morphological identification of Trichoderma spp. carried out using a trinocular microscope. There were six isolates analyzed based on internal transcribed spacer sequences (ITS) in ribosomal DNA region using PCR technique with ITS 1 and ITS 4. The results of this research showed that Trichoderma spp. original from PT BGA Central Kalimantan has diverse characteristics. Sequencing analysis showed that six isolates were in one group with Trichoderma asperellum isolates T5 (Acc. No. MH809176), IIPR-80 (Acc. No. MK841018) and TV5 (Acc. No. MH393299). Based on the antagonism test, it was found that six isolates were able to inhibit the growth of Ganoderma spp. with an average percentage value is ≥50%.