Coastal waters of Florida’s Big Bend, Gulf of Mexico (GOM) once supported one of the largest sea turtle fisheries in the United States. To fill an information gap in this region on abundance and distribution of sea turtles, we used vessel—based distance sampling and active capture methods to characterize current foraging aggregations near the St. Martins Marsh Aquatic Preserve. Over 10 sampling periods between 2012—2018, we completed 513 km of transects and recorded 819 turtles among 4 species—green turtle (Chelonia mydas, n = 624), Kemp’s ridley (Lepidochelys kempii, n = 147), loggerhead (Caretta caretta, n = 47), and a single hawksbill (Eretmochelys imbricata). Turtle densities in 4 study plots within the 200 km2 study site ranged from 57—221 immature green turtles/km2, 16—56 immature Kemp’s ridleys/km2, and 1—14 juvenile—to—adult loggerheads/km2. Of 200 green turtles captured, 67.5% showed skin tumors consistent with fibropapillomatosis, a frequency similar to that from urbanized estuaries of Florida’s east coast. The largest green turtles (> 60 cm straight standard carapace length), abundant in the southern portion of our study area, are of note because this size class is uncommonly recorded within US territorial waters. Analyses of green turtle mtDNA haplotypes found contributions from rookeries in the western GOM, Mexican Caribbean, and Costa Rica. Although Big Bend protected areas were principally designed to conserve marine and coastal habitats, these regulatory zones have also effectively encompassed a hotspot for foraging sea turtles.

Chelonid alphaherpesviruses 5 and 6 (ChHV5 and ChHV6) are viruses that affect wild sea turtle populations. ChHV5 is associated with the neoplastic disease fibropapillomatosis (FP), which affects green turtles (Chelonia mydas) in panzootic proportions. ChHV6 infection is associated with lung-eye-trachea disease (LETD), which has only been observed in maricultured sea turtles, although antibodies to ChHV6 have been detected in free-ranging turtles. To better understand herpesvirus prevalence and host immunity in various green turtle foraging aggregations in Florida, USA, our objectives were to compare measures of innate and adaptive immune function in relation to (1) FP tumor presence and severity, and (2) ChHV5 and ChHV6 infection status. Free-ranging, juvenile green turtles (N = 45) were captured and examined for external FP tumors in Florida’s Big Bend, Indian River Lagoon, and Lake Worth Lagoon. Blood samples were collected upon capture and analyzed for ChHV5 and ChHV6 DNA, antibodies to ChHV5 and ChHV6, in vitro lymphocyte proliferation using a T-cell mitogen (concanavalin A), and natural killer cell activity. Despite an overall high FP prevalence (56%), ChHV5 DNA was only observed in one individual, whereas 20% of turtles tested positive for antibodies to ChHV5. ChHV6 DNA was not observed in any animals and only one turtle tested positive for ChHV6 antibodies. T-cell proliferation was not significantly related to FP presence, tumor burden, or ChHV5 seroprevalence; however, lymphocyte proliferation in response to concanavalin A was decreased in turtles with severe FP (N = 3). Lastly, green turtles with FP (N = 9) had significantly lower natural killer cell activity compared to FP-free turtles (N = 5). These results increase our understanding of immune system effects related to FP and provide evidence that immunosuppression occurs after the onset of FP disease.

Assessing the distribution, density, and abundance of organisms is essential for conservation and management of imperiled species. Simple counts of sampled individuals are often inadequate to make such estimates. This is especially true for highly mobile marine animals like green sea turtles (Chelonia mydas). We used distance sampling and density surface model techniques to generate estimates of green turtle abundance on foraging grounds at the Eastern Quicksands, located west of Key West, Florida, USA. From 2006 to 2018, we conducted 18 surveys of six standardized transect lines from which we estimated abundances, plotted spatial distributions, and identified spatial segregation of life stages using density surface models and null model analysis. The Eastern Quicksands represent one of the densest foraging aggregations of green turtles worldwide. Spatial segregation of Large Juvenile and Adult turtles was evident, which we hypothesize may be due to benthic habitat preferences and differing predator detection and avoidance strategies among the sea turtle life stages. Given the high green turtle densities in this foraging area, and recent increases in Florida green turtle nesting, combined with anthropogenic stressors to seagrass pastures, we foresee management concerns for long-term sustainability of seagrass at the Eastern Quicksands and recommend that this area be given Critical Habitat designation.

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