No abstract

Reducing methane emissions from the oil and gas industry is a critical climate action policy tool in Canada and the US. Optical gas imaging-based leak detection and repair (LDAR) surveys are commonly used to address fugitive methane emissions or leaks. Despite widespread use, there is little empirical measurement of the effectiveness of LDAR programs at reducing long-term leakage, especially over the scale of months to years. In this study, we measure the effectiveness of LDAR surveys by quantifying emissions at 36 unconventional liquids-rich natural gas facilities in Alberta, Canada. A representative subset of these 36 facilities were visited twice by the same detection team: an initial survey and a post-repair re-survey occurring ∼0.5-2 years after the initial survey. Overall, total emissions reduced by 44% after one LDAR survey, combining a reduction in fugitive emissions of 22% and vented emissions by 47%. Furthermore, >90% of the leaks found in the initial survey were not emitting in the re-survey, suggesting high repair effectiveness. However, fugitive emissions reduced by only 22% because of new leaks that occurred between the surveys. This indicates a need for frequent, effective, and low-cost LDAR surveys to target new leaks. The large reduction in vent emissions is associated with potentially stochastic changes to tank-related emissions, which contributed ∼45% of all emissions. Our data suggest a key role for tank-specific abatement strategies as an effective way to reduce oil and gas methane emissions. Finally, mitigation policies will also benefit from more definitive classification of leaks and vents.

No abstract

Bacterial sepsis is a major global cause of death. However, the pathophysiology of sepsis has remained poorly understood. In industrialized nations, Staphylococcus aureus represents the pathogen most commonly associated with mortality due to sepsis. Because of the alarming spread of antibiotic resistance, anti-virulence strategies are often proposed to treat staphylococcal sepsis. However, we do not yet completely understand if and how bacterial virulence contributes to sepsis, which is vital for a thorough assessment of such strategies. We here examined the role of virulence and quorum-sensing regulation in mouse and rabbit models of sepsis caused by methicillin-resistant S. aureus (MRSA). We determined that leukopenia was a predictor of disease outcome during an early critical stage of sepsis. Furthermore, in device-associated infection as the most frequent type of staphylococcal blood infection, quorum-sensing deficiency resulted in significantly higher mortality. Our findings give important guidance regarding anti-virulence drug development strategies for the treatment of staphylococcal sepsis. Moreover, they considerably add to our understanding of how bacterial sepsis develops by revealing a critical early stage of infection during which the battle between bacteria and leukocytes determines sepsis outcome. While sepsis has traditionally been attributed mainly to host factors, our study highlights a key role of the invading pathogen and its virulence mechanisms.