No abstract

In this study, we investigated the effects of (-)-epigallocatechin-3-gallate (EGCG) on wound healing both in vitro and in vivo with and without infection. EGCG has antimicrobial properties and could be useful as a topical agent to prevent and/or treat wound infections. Normal fibroblasts were isolated from the dermis of C57BL/6 mice and cultured with 0, 0.001 to 0.400 mg/ml of EGCG. In vitro assays demonstrated that migration, proliferation, and apoptosis were inhibited at EGCG concentrations of 0.100-0.400 mg/ml. Expression of α-smooth muscle actin (α-SMA) was also reduced. In vivo experiments measured closure/contraction of full-thickness dorsal wounds that were treated with 0, 0.3, 3.0, and 30.0 mg/ml of EGCG every 24 hours. Macrophages (F4/80), neutrophils (Ly-6G) and myofibroblasts (α-SMA) were assessed at 48 and 168 hours. By 168 hours there was a significant reduction in presence with the 30 mg/ml dose vs. 0.3 and 3.0 mg/ml (p<0.009, p<0.006, respectively). The percentage of wound closure at one week in EGCG treated wounds was 87.87% (0.03 mg), 85.23% (0.3 mg) and 40.06% (30 mg/ml) compared to controls. Reduced quantities of α-SMA myofibroblasts were observed in the 3 mg EGCG treatment group compared to controls at 168 hours. We previously demonstrated that EGCG has antimicrobial properties (MIC 50 ~0.3 mg/ml). This data suggests that EGCG could potentially be applied to the wound surface as an antimicrobial without negatively influencing healing. To this end we applied EGCG (10 mg/ml) to a model of an infected traumatic wound. EGCG treatment significantly reduced bacterial load after one and two dose regimens.

Aim: Chronic osteomyelitis with pus discharge is very difficult to treat. This time we experienced the case of lower leg osteomyelitis, which we have treated with Trafermin spray. The result was excellent and beyond our expectation, so we will report this case.Case: 63 years, male. He sustained the open fracture injury of his right foot by a fallen rocks and resulted in the osteomyelitis. Then he was introduced to our hospital. Operation was done under spinal anesthesia, the infected bone and covering skin were excised fully and the defect (4 · 3 · 3 cm) was left open. After 2 weeks of dressing change, we began to use the Trafermin spray.Result: At the first 3 weeks, evident change was not recognized, but good granulation formation started 4 weeks later. This tendency has continued for a while and the defect completely closed 5 months later. Discussion:We have experienced the case of lower leg osteomyelitis, which showed the dramatic improvement. Through this case, we have reconfirmed that rich blood supply around the defect is essential for the healing process when we use the Trafermin spray.Aim: Taking advantage of homogeneously marked cells from green fluorescent protein (GFP) transgenic mice, we have previously demonstrated that bone marrow derived stromal cells (BSCs) differentiate into a variety of cell lineages both in vitro and in vivo. In the present study, we extend this approach to characterize adipose tissue derived stromal cells (ASCs), sometimes called processed lipoaspirate (PLA) cells.Methods: ASCs were isolated from inguinal fat pads of GFP mice. After the primary culture in control medium, the cells were incubated in osteogenic and chondrogenic medium for 2 to 4 weeks, respectively. Osteogenic and chondrogenic differentiation were assessed by the special staining and RT-PCR.Results: ASCs incubated in osteogenic medium were stained positively for von Kossa and alkaline phosphatase staining. RT-PCR analysis showed the Expression of osteocyte related genes. Positive staining cells for alcian blue and the expression of chodrocyte related genes were found in ASCs incubated in chondrogenic medium.Conclusions: These findings suggest that ASCs derived from GFP transgenic mice have both osteochondrogenic potential in vitro. Since this cell population can be easily identified through fluorescence microscope, it may be an ideal source of ASCs for further experiments of stem cell biology and tissue engineering.Aim: In the present study, expression of members of the fibroblast growth factor (FGF) family and early growth response gene 1 (Egr-1), known as a transcription factor, was analyzed by reverse transcription polymerase chain reaction (RT-PCR).Methods: After the mouse fetal small intestine was divided into single cells with collagenase-disperse, the cells were cultured. After reorganized small intestine were resected with a knife, expression of mRNAs from FGF family and Egr-1 was analyzed by RT-PCR.Results: The wound surface was covered with epithelial cells by 24 hours after resection.Among members of ...