Black birch fungus (Inonotus obliquusf sterillis, known in Russia as "chaga") is used in folk medicine for the treatment of gastrointestinal tract disorders. Since 1955, a dense extract of this fungus has been produced on the commercial level and used in medical practice as the drug befungin.The purpose of this work was to study the chemical composition and pharmacological properties of the total dry black birch fungus extract (DBFE), prepared according to a new patented technology [1], and the individual biologically active fractions.
EXPERIMENTAL CHEMICAL PARTFor the chemical analysis, a chromogenic polyphenol complex was isolated from DBFE by acidification with hydrochloric acid to pH 2.2-2.5. According to the published data [2,3], the polyphenol complex of DBFE was then hydrolyzed with a 10% hydrochloric acid solution in an autoclave at a pressure of 1.5 atm and a temperature of 126~ Neutral substances isolated from the hydrolyzate were used for the extraction of phenols and acids with chloroform in a neutral medium. Then acids were separated from phenols by using sodium bicarbonate, and flavonoids were isolated
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