Immobilized enzymes are now a significant and appropriate area of modern technologies. Immobilization of enzymes on nanoparticles (NPs) especially magnetic nanoparticles (MNPs) not only increase the stability of the enzymes by protecting the active site but also facilitates the separation mode. Immobilized technology is considered effective in context of running cost to exercise immobilized enzymes technique. Nowadays, variety of magnetic nanoparticles are available such as chitin-chitosan magnetic nanoparticles, Fe3O4 magnetic nanoparticles, bacteriophages T4 capsid novozym-435 etc. which are quite fit for loading enzymes and to use fruitfully. The main focus in this piece of work is that how immobilized enzymes are helpful in different biomedical uses and what kind of enzymes and nanoparticles could be hyphenated to take advantage in health care sectors. Different method of enzymes immobilization will also be discussed in details including both physical methods and chemical methods of loading enzymes on nanoparticles.