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In the present study is phytochemicals, trace metals and antimicrobial activity of ethanolic extract of Anacyclus pyrethrum. The phytochemical screening of the crude ethanolic root, stem and leaf extracts showed the positive results of steroids, triterpenes, reducing sugar, sugar, alkaloids, flavonoids, saponin, tannins, anthraquinones and amino acids. The average mean concentrations of Cd, Cr, Cu, Fe, Ni, Pb and Zn in plant sample were BDL, 0.03, 0.42, 0.79, BDL, BDL and 0.58 mg kg -1 , respectively. The descending order of the metal content in the plant sample were: Zn > Fe > Cu > Cd > Cr = Ni = Pb. Among various part of plant extracts studied for antimicrobial activity, root ethanolic extract showed highest of inhibition than leaves and stem ethanolic extracts.

Background: Multi-drug resistant gram positive bacteria that are common causes of hospital acquired infections are rapidly enhancing throughout the world Objectives: The present work aimed to examine pathogenic gram positive bacteria and their drug resistance profile. Materials and Methods: A total of 50 clinical urine specimens were collected and the pathogens were isolated and identified. Antibiotic sensitivity was evaluated by the Kirby bauer method. Results: Four strains of Staphylococcus aureus and five strains of Enterococcus faecium were isolated. Among the gram positive isolates, Staphylococcus aureus were highly resistant to ampicillin, ampicillin sulbactam, amoxy/clav, cefixime, penicillin, methicillin, cefepime and ceftazidime. The Enterococcus faecium strains were highly resistant to ampicillin, ampicillin sulbactam, methicillin, penicillin, cefotaxime, ceftazidime, cefpirome, cefuroxime, cefixime and trimethoprim and three strains of vancomycin. The multiple antibiotic indexes of these two strains were calculated as 0.428 for each strain. Conclusions: All the Staphylococcus aureus strains and Enterococcus faecium strains were sensitive to members of the oxazolidinone class of linezolid drug. Development of multi drug resistance by gram positive bacteria constitutes a very serious challenge for medical practice. Despite these rising rates, it is impossible to distinguish where they have originated from and special attention has thus been directed towards specific hospital surveillance systems and strict infection control measures for these microorganisms.