In the present study, phytochemical screening, content of total phenolics, antifungal and antioxidant activity of leaf and fruit extract of was investigated in vitro. The leaf and fruit extracts were obtained using methanol as solvent. The extracts were screened for phytochemicals by standard tests. Flavonoids, saponins, terpenoids, tannins and phenols were found in both extracts. Antifungal activity of extracts was evaluated by poisoned f growth of test fungi to high extent when compared to fruit extract. Antioxidant activity was determined by DPPH radical scavenging, ABTS radical scavenging and Ferric reducing assays. Both extracts exhibited radical scavenging and ferric reducing activity in dose dependent manner with marked activity exhibited by leaf extract. Folin method was employed to estimate total phenolic content of extracts. Phenolic content was slightly higher in leaf extract. A positive correlation was observed between phenolic content and antioxidant activity. The plant can be used against phytopathogenic fungi and to prevent and control oxidative damage. Further studies are to be carried out to isolate active principl from the plant and to determine their bioactivities.
The present study was conducted to determine antimicrobial and radical scavenging activity of Atylosia lineata Wt. & Arn. (Fabaceae). The shade dried and powdered leaves were extracted using methanol. Antibacterial and antifungal activity of extract was de diffusion and poisoned food technique respectively. Antiradical efficacy was determined by DPPH and ABTS radical scavenging assays. Total phenolic content of leaf extract was estimated by Folin-Ciocalteau Reagent method. The extract w bacteria with marked inhibitory effect against bacteria were susceptible to higher extent than Gram positive bacteria. The extract inhibited the growth of all test fungi to >50%. Among fun higher extent. The extract scavenged DPPH and ABTS radicals in dose dependent manner. At extract concentration 100µg/ml and higher, >90% scavenging of both radicals was observed. The total phenolic content of leaf extract was found to be 81.36mg GAE/g of extract. The plant appears promising to treat infectious diseases and oxidative damage.
The present study was performed to determine inhibitory effect of cow urine extract of 14 plants (belonging to 7 families) against the mycelial growth of two phytopathogenic fungi viz
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