A two-month-old German Shepherd pup was presented to Teaching Veterinary Clinical Complex, Mannuthy, Kerala with visible deformity of left forelimb. From the history narrated by the owner, the condition was congenital in occurrence.With the help of radiography, the condition was diagnosed as lobster claw syndrome.Due to the unwillingness of the owner for the surgical correction, we advised the palliative care.

Platelet rich plasma (PRP) therapy is an integral part of regenerative medicine as the platelets possess a good healing capacity owing to the presence of a wide variety of growth factors in the platelet granules found in the cytoplasm of the platelet. Autologous PRP was prepared from the blood of the patient itself, without any preservatives. Storage of PRP was one of the main hurdles of the treatment modality. During storage, the platelet counts may get reduced, undergo activation or get contaminated with bacteria as no preservatives are used in the preparation of autologous PRP. Cytological changes and microbial quality of the PRP during storage at 4oC and -20oC for seven days were analysed in this study. Reduction in platelet count and the chance of microbial contamination were less when autologous PRP was stored at -20°C compared to 4°C.