Odonate assemblages were compared between replicate sets of shallow lakes that had been created and acidified by open-cast mining across a large area (2,451 ha) of southwest France (Arjuzanx, Landes); one set of lakes (n 5 5) was experimentally restored by liming with calcium carbonate, whereas another group (n 5 5) was left as untreated reference lakes. Both odonate adults and exuviae were sampled bimonthly during MayAugust 1998. Elevated turbidity and conductivity in limed lakes were the only physicochemical measures differing between restored and reference lakes, because deacidification occurred naturally, even in reference lakes during the 17 years after the onset of restoration. Restoration by liming can apparently lead to effects on lake turbidity that might be considered adverse. Twenty-four and 19 odonate species occurred among adults and exuviae, respectively, but there were no significant differences in richness between restored and reference sites. However, significantly, more exuviae were collected from the reference sites (588 vs. 180), where exuvial diversity and rank abundance indicated more evenly structured assemblages than those in restored lakes. Ordination showed that adult assemblages differed significantly between restored and reference lakes, and varied highly significantly with lake turbidity. This effect occurred because a small group of generally scarce adults were characteristic of reference sites (Chalcolestes viridis, Lestes virens, Cordulia aenae, Leucorrhinia albifrons, and Sympetrum sanguineum). Exuviae of these same species were less abundant at restored sites, but exuvial assemblages overall did not discriminate between restored and reference lakes. We conclude that lake restoration by liming can reduce diversity and larval numbers among odonates and subtly affects adult assemblages. In this case study, adult assemblages discriminated best between the lake types involved in the experiment, but important additional information arose from exuvial abundance and structure. This study indicates that natural recovery processes after acidification in formerly open-cast areasrather than chemical intervention through limingmight lead to preferable conservation outcomes.

Ocean acidification (OA) and its subsequent changes in seawater carbonate chemistry are threatening the survival of calcifying organisms. Due to their use of calcium carbonate to build their shells, marine molluscs are particularly vulnerable. This study investigated the effect of CO2-induced OA on adult European abalone (Haliotis tuberculata) using a multi-parameter approach. Biological (survival, growth), physiological (pHT of haemolymph, phagocytosis, metabolism, gene expression), and structural responses (shell strength, nano-indentation measurements, Scanning electron microscopy imaging of microstructure) were evaluated throughout a 5-month exposure to ambient (8.0) and low (7.7) pH conditions. During the first 2 months, the haemolymph pH was reduced, indicating that abalone do not compensate for the pH decrease of their internal fluid. Overall metabolism and immune status were not affected, suggesting that abalone maintain their vital functions when facing OA. However, after 4 months of exposure, adverse effects on shell growth, calcification, microstructure, and resistance were highlighted, whereas the haemolymph pH was compensated. Significant reduction in shell mechanical properties was revealed at pH 7.7, suggesting that OA altered the biomineral architecture leading to a more fragile shell. It is concluded that under lower pH, abalone metabolism is maintained at a cost to growth and shell integrity. This may impact both abalone ecology and aquaculture.

Ocean acidification is a major global driver that leads to substantial changes in seawater carbonate chemistry, with potentially serious consequences for calcifying organisms. Marine shelled molluscs are ecologically and economically important species, providing essential ecosystem services and food sources for other species. Due to their physiological characteristics and their use of calcium carbonate (CaCO3) to build their shells, molluscs are among the most vulnerable invertebrates with regard to ocean acidification, with early developmental stages being particularly sensitive to pH changes. This study investigated the effects of CO2-induced ocean acidification on juveniles of the European abalone Haliotis tuberculata, a commercially important gastropod species. Six-month-old juvenile abalones were cultured for 3 months at four pH levels (8.1, 7.8, 7.7, 7.6) representing current and predicted near-future conditions. Survival, growth, shell microstructure, thickness and strength were compared across the four pH treatments. After three months of exposure, significant reductions in

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The increasing consumption of seafood products raises concerns over their sustainability and the conservation of marine resources. Seafood traceability, enabled by a regulated labelling system, is important to prevent overexploitation of these resources. The regulations (EU) No.1169/2011 and (EU) No 1379/2013 are the European legislative tools that specify the mandatory information that must be present on seafood labels. The present study analysed the labels of seafood products sold in different European countries in order to verify the presence of mandatory information required by EU regulations currently in place. The results show that there is a difference in compliance among groups of products and among countries. The country with the lowest level of compliance was The United Kingdom (still part of EU when the study was carried out), with an overall compliance of 63.7%. The country with the highest level of compliance was Portugal (87.2%). Across all the countries analysed, supermarkets were more compliant than fishmonger's shops and Processed Prepacked products were more conformed best to the EU labelling legislation when compared to Unprocessed Non-Prepacked products. Differences among different areas of the same country were also observed. Fishing gear, scientific name, fishing/production method and date of freezing were the types of information most frequently missing on the labels examined. The results of this study pose the bases for further actions, that can be taken by relevant institutions, to improve compliance throughout the supply chain.