Vanillin, a phenolic compound which is a major component of Vanilla planifolia, has been gaining interests due to its potential as natural food preservative and anti-oxidant. Vanillin is usually extracted from the pods of V. planifolia. This study deals with the detection of vanillin from different dried parts of in vitro and in vivo plant of V. planifolia, namely leaves, stem, and common root. The solvent used was ethyl acetate. The crude extract obtained was analyzed by using Thin Layer Chromatography (TLC) with a mixture of hexane and ethyl acetate with a volume ratio of 8:2 as the mobile phase and iodine vapors as the developing agent. The different parts of both in vivo and in vitro plant of Vanilla planifolia were found to contain vanillin. For sample concentration of 25 %, the R f values for the leaves, common root and stem of in vivo plant was 0.545, 0.539 and 0.532 respectively and for the in vitro plant was 0.532, 0.545, and 0.532. For sample oncentration of 50 %, the R f values for the leaves, common root and stem of in vivo plant was 0.469, 0.481 and 0.475 respectively and for the in vitro plant was 0.463, 0.475, and 0.494.

Stevia rebaudiana is an important sugar substitute belongs to Asteraceae family. The sweet taste of the species is contributed by stevioside, a natural sweetener extracted from the plant. This study is performed to detect the presence of stevioside in parts of S. rebaudiana plant and to determine the best solvent system in crude extraction of S. rebaudiana. Chemical extracts from dried shoots, leaves, stems and roots were attempted using three different extraction solvents which were hexane, methanol and water. Isolated stevioside was purified and analyzed by using Thin Layer Chromatography (TLC) method. The R f value obtained from 50% concentration of flowers, leaves, roots and shoots samples which were extracted with water were 0.41, 0.44, 0.41 and 0.41 respectively. While the R f value of 25% concentration of flowers, leaves, roots and shoots samples which were extracted with water were 0.35, 0.38, 0.39 and 0.28 respectively. From the results, we can conclude that stevioside is present in flowers, leaves, roots and shoots of S. rebaudiana plant and water is the best extraction solvent in stevioside extraction process.