Dans ce travail, la lutte chimique anti-larvaire a été réalisée au laboratoire chez deux espèces culicidiennes Anopheles hispaniola, vecteur potentiel du paludisme (Guy 1963), et Culex pipiens. Dans ces essais, nous avons utilisé le téméphos, larvicide organophosphoré, sous forme liquide 500 EC (Concentration Emultionnable). Dans ces conditions, un litre de produit commercial contient 500 g de produit actif (500 g.H).Cette étude a montré que la CL 50 (30mn) pour le stade L2 est de 21 ug.l" 1 chez AMspaniola et de 10 ug.H chez C. pipiens ; La CL 50 (60mn) au stade L4 est respectivement de 23 ug.H et 12 ug..H.Le taux de consommation des larves à'AMspaniola (stade L4) par Gambusia affinis varie en fonction des milieux étudiés mais reste toujours plus élevé chez les femelles que chez les mâles.
Efficiency of chemical and biological treatments on Culicidae larvae: lethal effect of temephos and consumption rate by
Gambusia affinisKeywords : chemical control, temephos (phosphoorganic), biological control, Gambusia affinis, malaria, Culicidae, Anopheles hispaniola, Culex pipiens.In this work, we carried out a laboratory study on chemical control of larvae of two species of Culicidae : Anopheles hispaniola, a potential vector of malaria (Guy 1963) and Culex pipiens using Temephos, an organo-phosphorus liquid-state EC500 (Emultion Concentation) larvicide. Under these conditions one litre of Temephos contains 500 g of active product (500 g.L -1 ). This study showed that the LC 50 (30min.) at L2 instar was 21 ug.l' 1 in A. hispaniola and 10 ug.H in C. pipiens. The LC 50 (60min.) at L4 instar was 23 ug.H and 12 ug.H respectively.Consumption rate of A.hispaniola larvae (instar L4) by the mosquitofish Gambusia affinis varies according to the media but remains higher in females than in males.
Les effets de trois métaux lourds (Cu, Hg, Cd) This study shows that A. hispaniola, which has a relatively short life cycle and is easy to rear in the laboratory, is twice as sensitive to mercury, 3.4 times as sensitive to cadmium and 8.6 times as sensitive to copper in comparison with Aedes aegypti. This latter species has been suggested as a test species for the same parameters to study the biological effects of heavy metals on aquatic insects.
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