An enzyme-linked immunosorbent assay (ELISA) was developed for the rapid quantitation of T-2 toxin, a tricothecene mycotoxin produced by members of the genus Fusarium. T-2 toxin was first converted to the T-2 hemisuccinate (T-2 HS) and then conjugated by the water-soluble carbodiimide method to either bovine serum albumin for use as an immunogen or to horseradish peroxidase for use as an enzyme marker. T-2 antiserum was air-dried onto polystyrene microtissue culture plates and the ELISA conducted by simultaneously incubating standards of T-2 toxin and the T-2 HS-peroxidase conjugate. Competition curves were prepared by determining total bound enzyme. The ELISA took about 2 hr to complete and allowed minimal detection of T-2 at levels of 2.5 pg/assay. Average recoveries from samples of wheat flour spiked with T-2 toxin in the 1.0-30 ppb range were 95 • 25% and those for corn meal spiked in the 5.0-30 ppb range were 98 + 19%. The results suggested the ELISA is a simple and convenient alternative for the screening ofT-2 toxin in food and feeds. 940A / JAOCS December 1981