Background: Hematological indices are important factors for the evaluations health status. Plant materials are often used as feed supplements by farmers to combat animal health challenges and have been a source of relief for the management of anemia in developing countries; for example, Whitfieldia lateritia is used in folklore medicine as anti-anemic agent. The hematopoietic, electrolyte and glycemic activities of Whitfieldia lateritia leaf decoction and vitali supplement on phenylhydrazine (PHZ)-induced anemic cockerels were investigated. Methods: A total of 60 cockerels were purchased and allowed to acclimatize for two weeks. Thereafter, classified into 12 groups (designated as T1….T12) of 5 cockerels each, where T2, T3 and T4 received 100, 200 and 400 mg/kg body weight W. lateritia leaf decoction, respectively. Anemia was induced in groups, T5……T12 by the administration of 10 mg/kg body weight PHZ for 3 consecutive days; thereafter, T5, T6 and T7 received 100, 200 and 400 mg/kg body weight W. lateritia leaf decoction, respectively; T8, T9 and T10 were administered 100, 200 and 400 mg/kg body weight vitali supplement, respectively; while T1, T11 and T12 were the normal control, negative control and baseline control, respectively. The treatment lasted for 30 days. Conclusion: Saponin, flavonoids, tannins, cardiac glycosides and steroids were detected. The glucose concentrations and hematological indices of the cockerels treated with the W. lateritia leaf decoction and the vitali supplement compared with the normal control. The W. lateritia leaf decoction displayed positive hematological, electrolyte and glycemic activities, thus may justify the use in maintaining animal health.