Blanched samples of frozen vegetable soybeans using peroxidase (POD) and lipoxygcnase (LIP) as blanching indices were compared with unblanched samples during storage by sensory evaluation, vitamin C determination and remaining enzyme activity. Samples using LIP and POD as blanching indices showed no significant differences in sensory quality during 160-day frozen storage, while both treatments were significantly different from unblanched samples. Vitamin C in samplcs using LIP as blanching index was higher than that in unblanched ones but not significantly different from that in samples using POD as an index. Samples using LIP as blanching index had 11.5% POD activity but no LIP activity, while blanched samples using POD as blanching index had no LIP or POD activities during storage.

This study investigated the effects of various concentrations and incubation time intervals of diallyl disulfide (DADS), the active principle of garlic, on: 1. cell viability, 2. lipid peroxidation, and 3. glutathione (GSH) concentration and its related enzyme activities of rat hepatocytes. According to the results of LDH leakage and microscopic examination, 0.5 and 1 mM DADS did not significantly affect the viability of hepatocytes. However, significant decrease in cell viability according to increased LDH leakage and significant changes in morphology of hepatocytes were observed at 2 mM DADS (P < 0.05). Lipid peroxidation was also detected when the hepatocytes were treated with 2 mM DADS. At 0.5 mM DADS, a higher GSH content was found in the hepatocytes although not at a statistically significant level. 0.5 and 1 mM DADS has little effect on the activities of glutathione-S-transferase (GST) and glutathione peroxidase (GPx); however a significant decrease in GST, GPx and glutathione reductase (GRd) activities was observed at 2 mM DADS. Once the media of 2 mM DADS was replaced with fresh medium at 24 hr treatment, the activities of GST, GRd and GPx were recovered, although they were still lower than the control values.