Feed enrichment with valuable nutrients for animals is a priority for the agricultural industry of Kazakhstan. This paper presents the technology of grain processing into feed preparation using lactic acid bacteria. Enzymatically hydrolyzed grain was a growing medium for the growth of lactic acid bacteria of the genus Lactobacillus. Cultivation of L.plantarum and L.salivarius bacteria in the saccharified grain hydrolysate helped to increase the total protein content in preparations by 26-45% and the enrichment of the feed preparation with glucose, lactic acid, and vitamins. This technology of grain processing into the feed preparation would be useful in terms of deep processing of grain and obtaining feed preparations containing important nutrient ingredients.

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Xylanases are hydrolytic enzymes involved in the degradation of xylan, the main component of plant biomass. In the present study xylanase, XynA, from Kazakh strain Bacillus licheniformis was obtained in recombinant form in Escherichia coli cells. The xynA gene was amplified from the genomic DNA of the B. licheniformis and cloned in pET-28c (+) vector under the control of the promoter of bacteriophage T7. Recombinant XynA was produced in ArcticExpressRP(DE3) cells by plasmid gene expression, protein purification was carried out by metal affinity chromatography. During the study, the dependence of the enzymatic activity of recombinant xylanase on temperature and pH was observed, it was established that xylanase has the highest activity at +50°C and pH of 6.9. The activity units at these values of temperature and pH were1859 per ml of purified protein. The working temperature and pH ranges in which xylanase conserves more than 70% activity from maximum is 40-60°C and 5-8, respectively. The data obtained are essential for the use of  carbohydrase xylanase from B.licheniformis in biotechnological processes in the processing of vegetable raw materials.