Phytophthora capsici is reported for the first time in the Lao PDR. It was isolated from soil collected from the root zone of wilting red chilli (Capsicum annuum) plants in a polyhouse farm in Paksong district of Champasak province. The wilting chilli plants had typical symptoms of infection by P. capsici including root rot and necrosis of the outer cortex of the stem base. The isolate was identified using morphological and molecular markers. A pathogenicity trial demonstrated that the culture was pathogenic to a local cultivar of chilli seedlings.
Yield decline associated with poor crop establishment, stunting, wilting, and diminished root systems was reported in processing tomato crops in Victoria, Australia. During surveys between 2016 and 2018 Pythium species were isolated by soil baiting and by culturing from the diseased roots and collars of plants exhibiting these symptoms. Eleven species of Pythium were identified based on cultural characteristics and phylogenetic analysis with ITS, Cox-1, and Cox-2 gene sequences. None of the 11 Pythium species had been reported previously from processing or fresh tomatoes in Australia. Pythium dissotocum was the most abundant and widespread species isolated during surveys in each of two growing seasons. In pathogenicity tests, these Pythium species ranged from nonpathogenic to highly aggressive. P. aphanidermatum, P. ultimum, and P. irregulare were consistently the most aggressive species, causing serious damage or death at the pregermination, postgermination, and later stages of plant growth. Five processing tomato cultivars varied significantly in their susceptibility to Pythium disease. These results suggest that Pythium species could be contributing to yield loss in processing tomatoes in Victoria both in the crop establishment phase and through the season.
Fusarium oxysporum f.sp. niveum is reported for the first time in the Lao PDR. It was isolated from watermelons (Citrullus lanatus) in Songkhon district, Savannahkhet province following a limited ad hoc survey during January and February of 2015. Infected plants showed symptoms of wilt, vascular discolouration, necrosis in the collar region, lower stem base and upper taproot regions, and whole plant death. Identification of the pathogen was confirmed through phylogenetic analysis of the EF1-α locus and a pathogenicity test satisfying Koch's postulates. Keywords Fusarium wilt. Citrullus lanatus. Watermelon Fusarium oxysporum f.sp. niveum (Fon) is the most economically significant pathogen of watermelons worldwide. Fon is host specific on watermelon and identification relies on
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