Micro-incisions were fabricated inside human cornea and sclera in vitro using single femtosecond laser pulses. In these experiments sclera was for the first time pre-cleared by means of a biocompatible and clinically safe (non-toxic) natural agent (refractive-index matching 40%-glucose solution in water), partially replacing water in the tissue comparing to its severe dehydration by previously used agents. Basic operational parameters of the corresponding microsurgical procedures are reported.

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