We demonstrate strain-induced coupling between a hole spin in a quantum dot and mechanical motion of a cantilever. The optical transitions of quantum dots integrated into GaAs mechanical resonators are measured synchronously with the motion of the driven resonators. In a Voigt magnetic field, both electron and hole spin splittings are measured, showing negligible change for the electron spin but a large change for the hole spin of up to 36%. This large effect is attributed to the stronger spin orbit interaction of holes compared to electrons.