The aim of this study was to investigate the Seroprevalence of brucellosis among the slaughtered food animals at Assiut Governorate abattoirs, Egypt. A total of 896 serum samples (747 from cattles & 149 from buffaloes) from different localities in Assiut Governorate abattoirs were tested for detection of the brucella antibodies. Results obtained by buffer acidified plate antigen test (BAPAT) and Rose Bengal plate test (RBT) as screening tests indicated a positive reactors percentage 2.19, 2.24, 2.14 and 4.0 % in Assiut, Abnoub, EL-Qusseia and Dairout respectively. The brucella positive reactors were subjected to confirmation by tube agglutination test (TAT) and Rivanol test (Riv.T). The present study revealed that RBT and BAPAT were the most sensitive as screening tests for diagnosis of brucella in slaughtered animals.