Opuntia ficus indica largely presents in Algerian rural environment, is frequently used by the local population for its therapeutic virtues.This particular study was carried out on Wistar albino rats, which were divided into four individual groups namely I, II, III and IV. The rats in Group I served as the control, received a distilled water, Group II was received thermoxidized vegetable oil, while Group III and IV were received thermoxidized oil with 100 and 500 mg/kg body weight of Opuntia ficus indica aqueous extract added in each respectively. At the end of 22 days administration, blood samples were collected for the analysis of lipid profile. The result showed that TG was significantly increased (P<0.05) in all the test groups when compared with the control, The HDL was significantly (P<0.05) increased in all the test samples except GroupII, while LDL followed a reverse trend. The phytochemical studies showed the presence of phytoconstituents like alkaloids, flavonoids and phenols.
A detailed study was performed on the antioxidant activity of the alcoholic extract of different plant parts of Cleome Arabica ssp. arabica (CAA) in relation with their total phenolic, flavonoid and proanthocyanidins contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. The total yield, total phenolic (TP), total flavonoids (TF) and total proanthocyanidins (TPA) contents of all the fractions were also determined. Cleome Arabica ssp. arabica was also subjected to preliminary phytochemical screening test for various constituents. The total phenolic contents (TPC) of three parts (Leaves, seeds and roots) was 78,56 ± 0,01 mg GAEq/g extract, while total flavonoid contents (TFC) of the same parts was 19,57 ± 0,01 mg REq/g extract, and total proanthocyanidins (TPA) was 17,41 ± 0,01 mg CYEq/ g of extract. Leaves extract was found significantly higher as compared to other parts extracts. Phytochemical screening of leaves, seeds and roots extracts revealed the presence of alkaloids, coumarins, flavonoids, saponins, tannins and terpenoids. European Scientific Journal April 2017 edition Vol.13, No.12 ISSN: 1857 -7881 (Print) e -ISSN 1857 103The antioxidant capacity of extracts was determined by three methods DPPH, FRAP, and phosphomolybdenum. The obtained results showed that leaves extract has the highest antioxidant capacity with low value of IC50 for DPPH and FRAP and the highest quantity of antioxydants compounds in phosphomolybdenum assay, followed by seeds extract and finally roots extract.
Our work is based on the phytochemical study, the antioxidant and the analgesic activities of a hydroalcoholic extract of Eruca vesicaria aerial part very used in traditional medicine. The phytochemical screening highlighted the presence of tannins, flavonoids, sterols, flavonoid glucosides, saponins, phenols and alkaloids. The determination of total phenols of hydromethanolic extract by the Folin-ciocalteu method revealed a content equal to 4.63 ± 0.017 mg GAE / g extract. The amount of flavonoids by AlCl3 method achieved a content equal to 2.14 ± 0.025 mg QE/ g extract. The antiradical activities were evaluated by two chemical tests ; DPPH and FRAP. The obtained results showed a high reducing activity. Besides, the acute toxicity and the analgesic activity of the plant extract was evaluated on rats, using a pain model induced by chemical stimuli, hot plate and tail immersion methods. The extract was used at various doses 100, 200 and 300 mg/kg, whereas acetylsalicylic acid was used as the standard drug (200 mg/kg). In the acetic acid-induced model, the plant extracts and the standard drug significantly (p<0.05) reduced the abdominal writhing in rats compared to the control group by increasing the percentage of writhing inhibition in a dose-dependent manner. In the hot plate and the tail immersion models, the plant extracts and the standard drug acetylsalicylic acid demonstrated a good analgesic activity and significantly (p<0.05) increasing the pain reaction time. These results indicate that the E. vesicaria plant has a significant analgesic potential that can act through peripheral and central mechanisms.
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