In the hibernating (diapausing) Colorado potato beetle, Leptinotarsa decemlineata Say, the flight muscles show pronounced degeneration. The muscle fibrils are greatly reduced in diameter and the sarcosomes are virtually absent. Similar signs of degeneration could be produced by extirpation of the postcerebral complex of endocrine glands, the corpora cardiaca and corpora allata. Reimplantation of active postcerebral complexes resulted in a very rapid regeneration of the muscle fibrils and new formation of sareosomes.
Scanning electron microscopy was used to examine microorganisms on the skin of cows' teats which were artificially contaminated and subsequently stored for different times. From the results it seems that bacteria were not spread uniformly on the surface of the teats. No extra-cellular polymers were observed before storage of the teats, but during storage polymers, in the form of thin fibers, were produced. These fibers became thicker and finally resulted in slime. Multiplication of bacteria during storage involved formation of microcolonies of bacteria in which bacteria were not attached directly to the skin but to other bacteria. This explains the decrease in the differences between bacterial counts obtained by the blending and rinsing methods after long periods of storage.
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