Introduction: The study was conducted in Mudumalai Tiger Reserve, in the Western Ghats to understand the effect of a single fire event on tree diversity and regeneration status. Four forest patches were selected which were unburned, 2-year-old burn, 5-year-old burn, and 15-year-old burn. Three 0.1 ha square plots were laid randomly in all four patches and analyzed for tree diversity, stand structure, and regeneration of tree species. Results: A total of 4129 individuals of tree species were recorded in field surveys, comprising 3474 seedlings, 121 saplings, and 534 trees. Totally, 40 tree species were recorded in study plots, from which 28 species were seedlings, 16 species were saplings, and 37 species were at tree stages. Conclusions: Tree diversity decreased in 2-year-old and 5-year-old burnt plots and was reached to the level of unburnt plots in 15 years of interval. Stems of small size classes started increasing after the fire. Seedling density increased linearly in subsequent years after fire but sapling and tree density recorded less than control in B2 but was higher in B5 and B15. The overall fire affected diversity, but regeneration showed a positive trend.
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