The biochemical composition of leaf gel isolated from vegetative and micropropagated Aloe vera plants at different stages of development had been compared. The total sugars, fructose, sucrose and starch were higher in micropropagated leaf gel at all developmental stages. The micropropagated plants have higher !-amylase activity at all stages with maximum at 6 month old. Catalase and peroxidase activities were higher in younger leaves (up to 4 month old) in both types of plants. The calcium and magnesium level increases up to maturity and ranges from 0.19 to 1.12% and 0.02 to 0.10% respectively, in conventionally grown plants whereas it was a little higher in micropropagated plants. Micropropagated plants had 12.5% higher phosphorus levels. The micropropagated plants (1 month old) have 60% higher phenol content as compared to conventional plants. The micropropagated mature plants have 55 and 18.6% higher saponin and sterol contents as compared to conventional plants. The composition of aloe vera gel depends upon the growth stages and method of propagation.