The soil sample was collected from the paddy field of Sriperumbudur, Tamilnadu which is having a history of repeated pesticide applications. The isolation of efficient pesticide degrading bacteria was identified as Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. The growth of the three pesticide degrading isolates was assessed in Minimal salt broth containing 25 ppm of pesticides. Two popularly used pesticides Metribuzin and Profenofos were selected for this study. Among the three bacterial isolates, the bacteria Bacillus subtilis utilized the pesticides effectively and showed maximum growth. The growth of the three pesticides degrading isolates Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis was assessed in Minimal salt broth containing 25 ppm of pesticides at different temperature levels (25 °C, 30 °C, 35 °C, 40 °C, 45 °C, 50 °C & 55 °C) and pH levels (pH 4, pH 5, pH 6, pH 7 & pH 8) and carbon sources (Lactose, Dextrose, Fructose, Mannose & Galactose) and nitrogen sources Peptone, Yeast extract, Beef extract, Malt extract and Casein respectively. The maximum growth rate of bacteria was recorded at 35 °C and pH 6. The maximum growth of bacteria was in the presence of Dextrose followed by Fructose, Galactose and Mannose. The least growth was recorded in Lactose broth culture. The maximum growth of bacteria was in the presence of Malt extract followed by Peptone, Yeast extract and Casein. The least growth was recorded in Beef extract broth culture. The bacterial isolates showed maximum growth in the Minimal salt broth containing Profenofos followed by Metribuzin.

A field trial was conducted to evaluate the residues of Bispyribac sodium 10 % SC on rice crop during Kharif season 2013 at Kandikai in Thiruvallur District, Tamilnadu, India. Randomized block design was followed withthree treatments forthree replicates. Bispyribac sodium 10 % SC 200 g a.i./ha, 500 g a.i./ha and control (water spray) was sprayed using hand operated Maax battery sprayer with a spray volume of 300 litres per hectare at 15 days after transplantation of rice crop (ADT 45). At harvest, samples of grain, straw and soil were collected replicate wise from each treatment along with the control. These samples were stored in icebox and transfer to the laboratory under cooled condition for analysis.All the residues samples were analyzed for Bispyribac sodium content by a validated HPLC method at the minimum detectable concentration of 0.01 ppm. The result revealed that no detectable level of Bispyribac sodium in straw, grain and soil at harvest. Thepost treatmentand preharvest intervalwas 60 days after transplantation.

Contribution/Originality: This study aimed to seek out the most antimicrobially active of the plants for testing and eventual use in medicine. INTRODUCTIONPlant remedies have been used for thousands of years to treat diseases and infections (Huffman, 2009). There is wide diversity of plants that have medicinal properties and this knowledge has been passed on through the generation (Estrada et al., 2011). The early man, when faced with various diseases, utilized the natural resources present around them to combat the symptoms and effects of various diseases (Mohanty & Pradhan, 2014). The primitive people characterized plants very simply based on what seemed to be good and useful versus what seemed not to be good and useful after trying them (Kunle, Egharevba, & Ahmadu, 2012). They tried various plants via application either topically or via ingestion as a concoction or by chewing to figure out what would cure/heal and

The soil sample was collected from the paddy field of Sriperumbudur, Tamilnadu which is having a history of repeated pesticide applications. The isolation of efficient pesticide degrading bacteria was identified as Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis. The growth of the three pesticide degrading isolates was assessed in Minimal salt broth containing 25 ppm of pesticides. Two popularly used pesticides Metribuzin and Profenofos were selected for this study. Among the three bacterial isolates, the bacteria Bacillus subtilis utilized the pesticides effectively and showed maximum growth. The growth of the three pesticides degrading isolates Pseudomonas aeruginosa, Staphylococcus aureus and Bacillus subtilis was assessed in Minimal salt broth containing 25 ppm of pesticides at different temperature levels (25 °C, 30 °C, 35 °C, 40 °C, 45 °C, 50 °C & 55 °C) and pH levels (pH 4, pH 5, pH 6, pH 7 & pH 8) and carbon sources (Lactose, Dextrose, Fructose, Mannose & Galactose) and nitrogen sources Peptone, Yeast extract, Beef extract, Malt extract and Casein respectively. The maximum growth rate of bacteria was recorded at 35 °C and pH 6. The maximum growth of bacteria was in the presence of Dextrose followed by Fructose, Galactose and Mannose. The least growth was recorded in Lactose broth culture. The maximum growth of bacteria was in the presence of Malt extract followed by Peptone, Yeast extract and Casein. The least growth was recorded in Beef extract broth culture. The bacterial isolates showed maximum growth in the Minimal salt broth containing Profenofos followed by Metribuzin

A field trial was conducted to evaluate the residues of Bispyribac sodium 10 % SC on rice crop during Kharif season 2013 at Kandikai in Thiruvallur District, Tamilnadu, India. Randomized block design was followed withthree treatments forthree replicates. Bispyribac sodium 10 % SC 200 g a.i./ha, 500 g a.i./ha and control (water spray) was sprayed using hand operated Maax battery sprayer with a spray volume of 300 litres per hectare at 15 days after transplantation of rice crop (ADT 45). At harvest, samples of grain, straw and soil were collected replicate wise from each treatment along with the control. These samples were stored in icebox and transfer to the laboratory under cooled condition for analysis.All the residues samples were analyzed for Bispyribac sodium content by a validated HPLC method at the minimum detectable concentration of 0.01 ppm. The result revealed that no detectable level of Bispyribac sodium in straw, grain and soil at harvest. Thepost treatmentand pre-harvest intervalwas 60 days after transplantation.