No abstract

The southeast Asian fluke Opisthorchis viverrini remains endemic, particularly in Thailand, Lao PDR, Cambodia, Vietnam, and Myanmar. However, there is a lack of data on the prevalence of liver fluke infection in Kratie Province in northeastern Cambodia. The present study aimed to detect O. viverrini DNA in fecal specimens using polymerase chain reaction (PCR) based on the internal transcribed spacer 2 (ITS2) region of ribosomal DNA (rDNA). The prevalence and percentage of O. viverrini infection were described by data analysis. The associated prevalence of O. viverrini infection was examined by bivariate binary logistic regression analyses. The total number of fecal specimens (n = 377) used for identifying food-borne trematodes was 6.89% of O. viverrini DNA-positive cases. Moreover, most O. viverrini infections were found in males (3.71%), those < 20 years (3.44%), those at primary school level (2.91%), farmers (3.18%), and the Trapaing Srae village (4.24%) in Snuol district, Kratie Province. O. viverrini infection was associated with age < 20 years (ORcru=0.569, 95% CI = 0.388–0.836, p = 0.004, ORadj=0.604, 95% CI = 0.412–0.885, p = 0.010). This study demonstrates that O. viverrini infection is distributed in rural areas located near freshwater reservoirs. Therefore, active surveillance, clinical examination of related hepatobiliary and cholangiocarcinoma, and health education are needed.

Background: Liver fluke, Opisthorchis viverrini, is associated to cholangiocarcinoma which is found frequently in some areas of Southeast Asian countries particularly in Thailand, Lao People Republic Democratic, Cambodia. This study sought to investigate the effects of an O. viverrini and cholangiocarcinoma (OVCCA) web application to facilitate health literacy regarding O. viverrini in Northeast Thailand. Methods: A randomized controlled trial study was performed among an intervention group (n=63) and a control group (n=63) during a one-year period from July 2019 to May 2020. The intervention group received the health literacy promotion program of O. viverrini information through the OVCCA web application for 6 weeks. The control group received an activity package from the public health department. The success of the program was evaluated at week 24 after the groups finished the last activity. ANCOVA, t-test and multiple logistic regression were used for data analysis for both groups. Results: The scores for knowledge; ability to access, understand, appraise, and apply information; motivation for protection; and practice of O. viverrini prevention were significantly higher for the intervention group than for the control group. The results indicated that a health literacy promotion program through an OVCCA web application could be advantageous for preventing and controlling O. viverrini infection. Conclusion: This intervention may be used as a potential strategy and guideline for self-care and health promotion in other endemic areas.