Brucellosis is one of the most common bacterial zoonosis in the world. It is caused by Brucella species and is an infectious and contagious disease transmissible to humans and to several animal species. This disease remains one of the neglected diseases in several countries and represents a real public health problem. A cross sectional study was conducted at the Port-Bouët abattoir in order to determine the seroprevalence as well as to assess the knowledge, attitudes and practices (KAPs) of workers. Three hundred and eighty-seven (387) cattle blood samples collected from January 5 to March 30 2019 were diagnosed using the Rose Bengal and indirect Enzyme-linked Immunosorbent Assay (i-ELISA) techniques. The seroprevalence was 0.52% (95% CI: 0.06265-1.8542) for i-ELISA and Rose Bengal. Regarding seroprevalence with sex, males recorded 0.3% (95 CI: 0.00776-1.6617), while females had 1.85% (95% CI: 0.04687-9.8991) for both Rose Bengal and i-ELISA tests with no statistically significant difference (X2=2; df=1; p=0.157). Animals >3years old recorded a higher seroprevalence rate eventhough with no statistically significant difference (X2=3; df=2; p=0.223). Also, our findings established that the potential risk of contracting brucellosis at the abattoir by workers is high due to the handling of animal tissues without the use of Personal Protective Equipments (PPEs). This baseline information indicates the neccesity for a more in-depth study on the traceability of animals coming to the abattoir as well as study the occurrence of brucellosis in animals and among abattoir workers following a one health approach in order to contribute to the development of a sub-regional integrated programme for the control of brucellosis. This collective approach will minimize the risk of contamination with brucellosis by workers.