ABSTRACT:The main aim of the study was to determine extracellular acid phosphomonoesterase activity in soil with and without addition of salicylic acid. Some other important chemical and physical features of soil were also investigated including the content and seasonal dynamics of bioavailable phosphorus and water soluble carbohydrate contents. Soil samples were collected at a depth of 5 cm (H horizon) at the Rájec-Němčice Ecosystem Station (southern Moravia, Czech Republic) in three differently managed Norway spruce pure stands. No correlation was found between the acid phosphomonoesterase activity and phosphorus content. The addition of salicylic acid reduced the acid phosphomonoesterase activity compared to soil without any substrate added.