Aim: The aim is to experimentally verify the methodology of self-regulation culture formation as an important element of professional training of future teachers of the humanities in the process of studying the author’s specialized course referred to as “Fundamentals of Psychovaleology”. Materials and Methods: The research involved third-year students of pedagogical institutions of higher education aged 20 to 23 years in the number of 126 people (80 girls and 46 boys). The methods used included observation, questionnaires, psychodiagnostic methods for determining the level of anxiety, self-assessment of well-being, quantitative and qualitative analysis of the results in terms of self-regulation culture formation according to theoretical, practical and professional criteria. Results: Experimental verification of the effectiveness of the developed methodology shows qualitative positive changes in the students’ emotional well-being, in significant reduction of their anxiety, as well as in increasing motivation for active exercise Conclusions: The developed methodology of self-regulation culture formation in future teachers can become a significant basis for the further formation of individual health preservation competence of students i. e. future teachers, their orientation to active physical education as well as fitness and health recreation activities, and can also be included in the system of implementation of professional standards for the training of students in pedagogical specialties, especially in terms of the development of their health preservation competence

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