Global polarization of Λ hyperons has been measured to be of the order of a few tenths of a percent in Au+Au collisions at √ s N N = 200 GeV, with no significant difference between Λ andΛ.These new results reveal the collision energy dependence of the global polarization together with the results previously observed at √ s N N = 7.7 -62.4 GeV and indicate noticeable vorticity of the medium created in non-central heavy-ion collisions at the highest RHIC collision energy. The signal is in rough quantitative agreement with the theoretical predictions from a hydrodynamic model and from the AMPT (A Multi-Phase Transport) model. The polarization is larger in more peripheral collisions, and depends weakly on the hyperon's transverse momentum and pseudorapidity η H within |η H | < 1. An indication of the polarization dependence on the event-by-event charge asymmetry 3 is observed at the 2σ level, suggesting a possible contribution to the polarization from the axial current induced by the initial magnetic field. PACS numbers: 25.75.-q, 25.75.Ld

Efficient and specific host cell entry is of exquisite importance for intracellular pathogens. Parasites of the phylum Apicomplexa are highly motile and actively enter host cells. These functions are mediated by type I transmembrane invasins of the TRAP family that link an extracellular recognition event to the parasite actin-myosin motor machinery. We systematically tested potential parasite invasins for binding to the actin bridging molecule aldolase and complementation of the vital cytoplasmic domain of the sporozoite invasin TRAP. We show that the ookinete invasin CTRP and a novel, structurally related protein, termed TRAP-like protein (TLP), are functional members of the TRAP family. Although TLP is expressed in invasive stages, targeted gene disruption revealed a nonvital role during life cycle progression. This is the first genetic analysis of TLP, encoding a redundant TRAP family invasin, in the malaria parasite.The phylum Apicomplexa consists of unicellular eukaryotes, such as Plasmodium and Toxoplasma gondii, that are obligate intracellular parasites in a wide range of invertebrate and vertebrate hosts, including humans. Despite vast differences in host range and target cell specificity, these parasites share a unique mechanism of active actin-dependent motility and host cell entry (18,27). Gliding locomotion and successful host cell entry through simultaneous formation of a parasitophorous vacuole are rapid processes and are thought to be mechanistically coupled (21). Both functions are mediated by the parasite's actin-myosin motor machinery (6, 22) and additional proteins, including type I transmembrane proteins of the TRAP/MIC2 family of invasins (12,29,30), the invasin bridging protein aldolase (4,14), and the myosin tail interacting protein MTIP (3). According to the current model (1, 26), an extracellular recognition event results in connection of the transmembrane invasins to short actin polymers that in turn are rapidly pulled backwards by immobilized motor myosins.So far, only TRAP-like invasins have been shown to act directly in parasite locomotion and invasion. In two invasive stages of the Plasmodium parasite, sporozoites and ookinetes, thrombospondin-related anonymous protein (TRAP) and circumsporozoite-and TRAP-related protein (CTRP), respectively, fulfill these functions (5, 29, 30). These proteins share a unifying primary structure, i.e., combinations of two adhesive modules, the von Willebrand factor A-domain (A domain) (37) and the thrombospondin type I repeat (TSR) (33), in their ectodomains, a transmembrane domain (25) and a cytoplasmic tail domain (CTD) (16). Importantly, the CTD of the sporozoite invasin TRAP is essential for gliding motility and cell entry of Plasmodium sporozoites, since a carboxy-terminal truncation of Plasmodium berghei TRAP resulted in noninvasive sporozoites. This finding also permitted a functional assay, and it was demonstrated that the CTD of the T. gondii tachyzoite invasin MIC2 could rescue the loss-of-function mutant (16). This complementation experime...

The first evidence of spin alignment of vector mesons (K Ã0 and ϕ) in heavy-ion collisions at the Large Hadron Collider (LHC) is reported. The spin density matrix element ρ 00 is measured at midrapidity (jyj < 0.5) in Pb-Pb collisions at a center-of-mass energy (ffiffiffiffiffiffiffi ffi s NN p) of 2.76 TeV with the ALICE detector. ρ 00 values are found to be less than 1=3 (1=3 implies no spin alignment) at low transverse momentum (p T < 2 GeV=c) for K Ã0 and ϕ at a level of 3σ and 2σ, respectively. No significant spin alignment is observed for the K 0 S meson (spin ¼ 0) in Pb-Pb collisions and for the vector mesons in pp collisions. The measured spin alignment is unexpectedly large but qualitatively consistent with the expectation from models which attribute it to a polarization of quarks in the presence of angular momentum in heavy-ion collisions and a subsequent hadronization by the process of recombination.

The ALICE Collaboration has measured the energy dependence of exclusive photoproduction of J/ψ vector mesons off proton targets in ultra-peripheral p-Pb collisions at a centre-of-mass energy per nucleon pair √ s NN = 5.02 TeV. The e + e − and μ + μ − decay channels are used to measure the cross section as a function of the rapidity of the J/ψ in the range −2.5 < y < 2.7, corresponding to an energy in the γ p centre-of-mass in the interval 40 < W γ p < 550 GeV. The measurements, which are consistent with a power law dependence of the exclusive J/ψ photoproduction cross section, are compared to previous results from HERA and the LHC and to several theoretical models. They are found to be compatible with previous measurements.

The objective of this study was to develop a sustained release dosage form of Trimetazidine dihydrochloride (TMZ) using a natural polymeric carrier prepared in a completely aqueous environment. TMZ was entrapped in calcium alginate beads prepared with sodium alginate by the ionotropic gelation method using calcium chloride as a crosslinking agent. The drug was incorporated either into preformed calcium alginate gel beads (sequential method) or incorporated simultaneously during the gelation stage (simultaneous method). The beads were evaluated for particle size and surface morphology using optical microscopy and SEM, respectively. Beads produced by the sequential method had higher drug entrapment. Drug entrapment in the sequential method was higher with increased CaCl2 and polymer concentration but lower with increased drug concentration. In the simultaneous method, drug entrapment was higher when polymer and drug concentration were increased and also rose to a certain extent with increase in CaCl2 concentration, where further increase resulted in lower drug loading. FTIR studies revealed that there is no interaction between drug and CaCl2. XRD studies showed that the crystalline drug changed to an amorphous state after formulation. Release characteristics of the TMZ loaded calcium alginate beads were studied in enzyme-free simulated gastric and intestinal fluid.
O objetivo deste estudo foi desenvolver forma de liberação controlada de dicloridrato de trimetazidina (TMZ) utilizando transportador plomérico natural em ambiente completamente aquoso. A TMZ foi presa em pérolas de alginato de cálcio preparadas com alginato de sódio pelo método de gelatinização ionotrópica, usando cloreto de cálcio como agente de formação de ligações cruzadas. O fármaco foi incorporado nas pérolas de gel de alginato de cálcio (método sequencial) ou incorporado, simultaneamente, durante o estágio de gelificação (método simultâneo). As pérolas foram avaliadas quanto ao tamanho das partículas e morfologia da superfície utilizando microscopia óptica de SEM, respectivamente. As pérolas produzidas pelo método sequencial apresentaram maior capacidade de inclusão. No método sequencial, a inclusão de fármaco foi maior com o aumento de CaCl2 e da concentração do plímero, mas menor com o aumento da concentração de fármaco. No método simultâneo, a inclusão de fármaco foi mais alta quando as concentrações de fármaco e plímero foram aumentadas e, também, atingiram certa extensão com aumento na concentração de CaCl2, cujo aumento posterior resultou em carga menor de fármaco. Estudos de FTIR revelaram que não há interação entre fármaco e CaCl2. Estudos de XRD mostraram que o fármaco mudou do estado cristalino para o amorfo após a formulação. As características de liberação de TMZ das pérolas carregadas com alginato de cálcio foram estudadas em fluidos simulados, gástrico e intestinal, livres de enzima