The aim of our work was to survey private farms in the Poltava region on the prevalence of Chlamydia-like organisms Waddlia chondrophila and Parachlamydia acanthamoebae. Materials and methods: The PCR research was conducted in 2018-2019 at the Institute of Pig Breeding and Аgro-industrial production of the NAAS. The study materials were 300 vaginal scrapings of cows and 28 samples of clinical material of pigs and boars with a suspicion of a chlamydial infection, which were selected at private farms in the Poltava region. Results: Chlamydia-like bacteria Waddlia chrondophila DNA was detected in 132 (44%) samples taken from cows and 8 (28.6%) samples from pigs and boars. Parachlamydia acanthamoebae DNA was not detected in pig samples. However, 43 (14.3%) clinical samples taken from cows were found to be Parachlamydia acanthamoebae – positive. 9 (3%) cows and 2 (7.14%) pigs turned out to be Chlamydia spp. – positive (the samples contained DNA of bacteria of the family Chlamydiaceae). Сonclusions: Our data indicate a relatively high prevalence of Chlamydia-like bacteria in problematic as to chlamydial infection farms in the Poltava region. Chlamydial infection carry a zoonotic threat, therefore it is necessary to observe safety and personal hygiene measures when working with animals. In addition, it is necessary to carry out periodic screening among the population with reproductive disorders and in occupational risk groups, periodic monitoring in livestock farms, to avoid the consuming the unpasteurized milk, and thermally unprocessed meat.

Introduction: Waddlia chondrophila and Parachlamydia acanthamoebae are well-known and best-studied representatives of Сhlamydia-related bacteria carrying a potential zoonotic threat. These bacteria are associated with miscarriage, ectopic pregnancy, diseases of the respiratory system in both humans and animals. Despite the importance of these Сhlamydia-like organisms for human medicine along with veterinary medicine, studies on their prevalence in Ukraine were not conducted due to the lack of available tests. The aim of our work was to create relatively cheap and easy method for detection Waddlia chondrophila and Parachlamydia acanthamoebae. Materials and methods: GenBank database was used to find nucleotide sequences of the 16S rRNA gene of bacteria Chlamydiales’ order. Alignment was performed using the MEGA7 software, in order to detect the presence of polymorphic hybridization sites specifically attributed to Waddlia chondrophila and Parachlamydia acanthamoebae. Primer- BLAST software was used to design oligonucleotide primers, to evaluate the critical parameters of the primer, in particular, the melting temperature, difference between melting temperatures for the primer pairs, the GC content, the self-complementarity, etc. Results and conclusions: The amplification of control DNA of Parachlamydia acanthamoebae and Waddlia chrondophila in single PCR using the corresponding primers and subsequent gel electrophoresis of PCR products determined the size of the amplified DNA fragments 88 b.p. and 123 b.p, respectively; the fragments were in line with the expected sizes. The analytical specificity test was performed by amplifying the control DNA of 15 species of the order Chlamydiales.

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