Background: Transfusion of plateletpheresis is effective for treating patients with platelet related insufficiencies while limiting recipient'sexposure to platelets from multiple donors. A larger yield is obtained when donor has a greater number of initial platelets and separation done in a shorter time. The aim of the study was to analyze the effect of various donors and procedure related parameters on the yield of single donor platelet so that blood donors can be screened effectively in less time. Materials and Methods:One thousand and one hundred plateletpheresis procedures were performed in a tertiary-care super-specialty hospital onFenwal AMICUS machine using closed system kits. Statistical analysis was done to establish cause and effect relationship and to find the extent of relationship between different variables.Results: Height, weight,BMI, platelet count and total leucocyte count had significant correlation with platelet yield, processing time and blood volume processed while haemoglobin especially in females correlated significantlywith processing time(p<0.0001)and blood volume processed. Rh "negative" donors had shorter processing time than Rh "positive" donors while ABO group did not show any correlation with machine variables. Conclusion:Platelet yield correlated positively with pre-donation count, height, weight and BMI. Haemoglobin correlated with processing time and blood volume processed. Optimization of platelet yield is an emerging issue to identify factors which may help in selecting donors to obtain higher platelet yields in shorter time and consequently better clinical outcome.